MYT1L Protein (AA 1-1187) (Strep Tag)
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- 抗原 See all MYT1L products
- MYT1L (Myelin Transcription Factor 1-Like (MYT1L))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1187
- 宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This MYT1L protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MDVDSEEKRH RTRSKGVRVP VEPAIQELFS CPTPGCDGSG HVSGKYARHR SVYGCPLAKK RKTQDKQPQE PAPKRKPFAV KADSSSVDEC YESDGTEDMD DKEEDDDEEF SEDNDEQGDD DDEDEVDRED EEEIEEEDDE EDDDDEDGDD VEEEEEDDDE EEEEEEEEEE NEDHQMSCTR IMQDTDKDDN NNDEYDNYDE LVAKSLLNLG KIAEDAAYRA RTESEMNSNT SNSLEDDSDK NENLGRKSEL SLDLDSDVVR ETVDSLKLLA QGHGVVLSEN ISDRSYAEGM SQQDSRNMNY VMLGKPMNNG LMEKMVEESD EEVCLSSLEC LRNQCFDLAR KLSETNPQDR SQPPNMSVRQ HVRQEDDFPG RTPDRSYSDM MNLMRLEEQL SPRSRTFSSC AKEDGCHERD DDTTSVNSDR SEEVFDMTKG NLTLLEKAIA LETERAKAMR EKMAMDAGRR DNLRSYEDQS PRQLAGEDRK SKSSDSHVKK PYYGKDPSRT EKRESKCPTP GCDGTGHVTG LYPHHRSLSG CPHKDRVPPE ILAMHENVLK CPTPGCTGRG HVNSNRNSHR SLSGCPIAAA EKLAKAQEKH QSCDVSKSNQ ASDRVLRPMC FVKQLEIPQY GYRNNVPTTT PRSNLAKELE KYSKTSFEYN SYDNHTYGKR AIAPKVQTRD ISPKGYDDAK RYCKNASPSS STTSSYAPSS SSNLSCGGGS SASSTCSKSS FDYTHDMEAA HMAATAILNL STRCREMPQN LSTKPQDLCT ARNPDMEVDE NGTLDLSMNK QRPRDSCCPV LTPLEPMSPQ QQAVMSSRCF QLSEGDCWDL PVDYTKMKPR RVDEDEPKEI TPEDLDPFQE ALEERRYPGE VTIPSPKPKY PQCKESKKDL ITLSGCPLAD KSIRSMLATS SQELKCPTPG CDGSGHITGN YASHRSLSGC PRAKKSGIRI AQSKEDKEDQ EPIRCPVPGC DGQGHITGKY ASHRSASGCP LAAKRQKDGY LNGSQFSWKS VKTEGMSCPT PGCDGSGHVS GSFLTHRSLS GCPRATSAMK KAKLSGEQML TIKQRASNGI ENDEEIKQLD EEIKELNESN SQMEADMIKL RTQITTMESN LKTIEEENKV IEQQNESLLH ELANLSQSLI HSLANIQLPH MDPINEQNFD AYVTTLTEMY TNQDRYQSPE NKALLENIKQ AVRGIQV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- MYT1L (Myelin Transcription Factor 1-Like (MYT1L))
- 别名
- Myt1l (MYT1L 产品)
- 别名
- 2900046C06Rik Protein, 2900093J19Rik Protein, C630034G21Rik Protein, Nztf1 Protein, Pmng1 Protein, Png-1 Protein, mKIAA1106 Protein, Nzf-1 Protein, Nzf1 Protein, NZF1 Protein, ZC2HC4B Protein, si:dkey-260n20.1 Protein, myelin transcription factor 1-like Protein, myelin transcription factor 1 like Protein, myelin transcription factor 1-like, a Protein, Myt1l Protein, MYT1L Protein, myt1la Protein
- 背景
- Myelin transcription factor 1-like protein (MyT1-L) (MyT1L) (Neural zinc finger factor 1) (NZF-1) (Postmitotic neural gene 1 protein) (Zinc finger protein Png-1),FUNCTION: Transcription factor that plays a key role in neuronal differentiation by specifically repressing expression of non-neuronal genes during neuron differentiation (PubMed:28379941). In contrast to other transcription repressors that inhibit specific lineages, mediates repression of multiple differentiation programs (PubMed:28379941). Also represses expression of negative regulators of neurogenesis, such as members of the Notch signaling pathway, including HES1 (PubMed:28379941). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220). Directly binds the 5'-AAGTT-3' core motif present on the promoter of target genes and represses transcription by recruiting a multiprotein complex containing SIN3B (PubMed:28379941). The 5'-AAGTT-3' core motif is absent from the promoter of neural genes (PubMed:28379941). {ECO:0000269|PubMed:20107439, ECO:0000269|PubMed:24243019, ECO:0000269|PubMed:27281220, ECO:0000269|PubMed:28379941, ECO:0000269|PubMed:9130664, ECO:0000269|PubMed:9373037}.
- 分子量
- 132.9 kDa
- UniProt
- P97500
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