CIITA Protein (AA 1-1155) (Strep Tag)
-
- 抗原 See all CIITA 蛋白
- CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1155
- 宿主
- 小鼠
-
资源
- Tobacco (Nicotiana tabacum)
- 标记
- This CIITA protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
-
MNHFQAILAQ VQTLLSSQKP RQVRALLDGL LEEELLSREY HCALLHEPDG DALARKISLT LLEKGDLDLT FLSWVCNSLQ APTVERGTSY RDHGDHSLCA TMDLGSPEGS YLELLNSDAD PLHLYHLYDQ MDLAGEEEIE LSSEPDTDTI NCDQFSKLLQ DMELDEETRE AYANIAELDQ YVFQDTQLEG LSKDLFIEHI GAEEGFGENI EIPVEAGQKP QKRRFPEEHA MDSKHRKLVP TSRTSLNYLD LPTGHIQIFT TLPQGLWQIS GAGTGLSSVL IYHGEMPQVN QVLPSSSLSI PSLPESPDRP GSTSPFTPSA ADLPSMPEPA LTSRVNETED TSPSPCQEGP ESSIKLPKWP EAVERFQHSL QDKYKALPQS PRGPLVAVEL VRARLERGSN KSQERELATP DWTERQLAHG GLAEVLQVVS DCRRPGETQV VAVLGKAGQG KSHWARTVSH TWACGQLLQY DFVFYVPCHC LDRPGDTYHL RDLLCPPSLQ PLAMDDEVLD YIVRQPDRVL LILDAFEELE AQDGLLHGPC GSLSPEPCSL RGLLAGIFQR KLLRGCTLLL TARPRGRLAQ SLSKADAIFE VPSFSTKQAK TYMRHYFENS GTAGNQDKAL GLLEGQPLLC SYSHSPVVCR AVCQLSKALL EQGTEAQLPC TLTGLYVSLL GPAAQNSPPG ALVELAKLAW ELGRRHQSTL QETRFSSVEV KTWAVTQGLM QQTLETTEAQ LAFSSFLLQC FLGAVWLAQC NEIKDKELPQ YLALTPRKKR PYDNWLEGVP RFLAGLVFQP RAHCLGALVE PAVAAVADRK QKVLTRYLKR LKLGTLRAGR LLELLHCAHE TQQPGIWEHV AHQLPGHLSF LGTRLTPPDV YVLGRALETA SQDFSLDLRQ TGVEPSGLGN LVGLSCVTSF RASLSDTMAL WESLQQQGEA QLLQAAEEKF TIEPFKAKSP KDVEDLDRLV QTQRLRNPSE DAAKDLPAIR DLKKLEFALG PILGPQAFPT LAKILPAFSS LQHLDLDSLS ENKIGDKGVS KLSATFPQLK ALETLNLSQN NITDVGACKL AEALPALAKS LLRLSLYNNC ICDKGAKSLA QVLPDMVSLR VMDVQFNKFT AAGAQQLASS LQKCPQVETL AMWTPTIPFG VQEHLQQLDA RISLR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product CIITA 蛋白
-
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))
- 别名
- Ciita (CIITA 产品)
- 别名
- CIITA Protein, C2ta Protein, EG669998 Protein, Gm9475 Protein, C2TA Protein, CIITAIV Protein, MHC2TA Protein, NLRA Protein, Mhc2ta Protein, class II, major histocompatibility complex, transactivator Protein, class II major histocompatibility complex transactivator Protein, MHC class II transactivator Protein, class II transactivator Protein, CIITA Protein, LOC710761 Protein, ciita Protein, LOC100443575 Protein, Ciita Protein, LOC100736732 Protein
- 背景
- MHC class II transactivator (CIITA) (EC 2.3.1.-) (EC 2.7.11.1),FUNCTION: Essential for transcriptional activity of the HLA class II promoter, activation is via the proximal promoter (PubMed:37327784). Does not bind DNA (By similarity). May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both (By similarity). Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter (By similarity). Also mediates enhanced MHC class I transcription, the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes (By similarity). Activates CD74 transcription (PubMed:32855215). Exhibits intrinsic GTP-stimulated acetyltransferase activity (By similarity). Exhibits serine/threonine protein kinase activity: phosphorylates the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (By similarity). {ECO:0000250|UniProtKB:P33076, ECO:0000269|PubMed:32855215, ECO:0000269|PubMed:37327784}.
- 分子量
- 127.5 kDa
- UniProt
- P79621
- 途径
- Cancer Immune Checkpoints
-