Sestrin 2 Protein (SESN2) (AA 1-480) (Strep Tag)
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- 抗原 See all Sestrin 2 (SESN2) 蛋白
- Sestrin 2 (SESN2)
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-480
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This Sestrin 2 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
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MIVADSECHS EIKGYLPFTR GGVAGPETRE EHREGQARRG SRGPSAFIPV EEILREGAES LEQHLGLEAL MSSGRVDNLA VVMGLHPDYL SSFWRLHYLL LHTDGPLASS WRHYIAIMAA ARHQCSYLVG SHMTEFLQTG GDPEWLLGLH RAPEKLRKLS EVNKLLAHRP WLITKEHIQA LLKTGEHSWS LAELIQALVL LTHCHSLASF VFGCGILPEG DAEGSPASQA PSPPSEQGTP PSGDPLNNSG GFEAARDVEA LMERMRQLQE SLLRDEGASQ EEMENRFELE KSESLLVTPS ADILEPSPHP DILCFVEDPA FGYEDFTRRG TQAPPTFRAQ DYTWEDHGYS LIQRLYPEGG QLLDEKFQVA CSLTYNTIAM HSGVDTSMLR RAIWNYIHCV FGIRYDDYDY GEVNQLLERN LKIYIKTVAC YPEKTTRRMY NLFWRHFRHS EKVHVNLLLL EARMQAALLY ALRAITRYMT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product SESN2 蛋白
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Sestrin 2 (SESN2)
- 别名
- Sesn2 (SESN2 产品)
- 别名
- HI95 Protein, SES2 Protein, SEST2 Protein, Ses2 Protein, MGC81239 Protein, SESN2 Protein, sestrin-2 Protein, hi95 Protein, ses2 Protein, sest2 Protein, Sestrin-2 Protein, RGD1566319 Protein, sestrin 2 Protein, sestrin 2 L homeolog Protein, SESN2 Protein, Sesn2 Protein, sesn2.L Protein, sesn2 Protein
- 背景
- Sestrin-2 (EC 1.11.1.-),FUNCTION: Functions as an intracellular leucine sensor that negatively regulates the mTORC1 signaling pathway through the GATOR complex (PubMed:18692468, PubMed:25259925). In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents mTORC1 signaling (PubMed:18692468, PubMed:25259925). Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:18692468, PubMed:25259925). This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses (By similarity). May negatively regulate protein translation in response to endoplasmic reticulum stress, via mTORC1 (PubMed:24947615). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed:18692468). Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1. However, this could not be confirmed (By similarity). {ECO:0000250|UniProtKB:P58004, ECO:0000269|PubMed:18692468, ECO:0000269|PubMed:23274085, ECO:0000269|PubMed:24947615, ECO:0000269|PubMed:25259925}.
- 分子量
- 54.4 kDa
- UniProt
- P58043
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