POLG Protein (AA 1-1218) (Strep Tag)
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- 抗原 See all POLG 蛋白
- POLG (Polymerase (DNA Directed), gamma (POLG))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1218
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This POLG protein is labelled with Strep Tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- 序列
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MSRLLWKKVA GAKVASGPVP ATARWVASSV LDPVPSDGRP PSQMPSSENG QLRLNPLLIQ MLSRGLHEQI FGCGGEMPDE AAVQRSVEHL QKHGLWGQPA TPLPDVELRL PRLFGGNLDQ HFRLLAQKQS LPYLEAAASL LEAQLPPEPK SWAWAEGWTR YGPEGEAEPV AIPEERALVF DVEVCLAEGT CPTLAVAISP SAWYSWCSRR LVEERYSWTS QLSPADLIPL GGSTSASSST KQDGQEQLVV GHNVSFDRAH IREQYLIQDS RMRFLDTMSM HMAISGLSSF QRSLWMGAKQ GKHKNPAAHK ARAEVPEESQ WSSESSSWDW MDISSANNLA DVHNLYVGGP PLEKEPRELF VKGSMRDIRE NFQDLMQYCA RDVWATFEVF QQQLPLFLER CPHPVTLAGM LEMGVSYLPV NQNWERYLTE AQNTYEELQR EMKKSLMDLA NDACQLLSGE RYKEDPWLWD LEWDLQEFKQ KKAKKVKKPA SASKLPIEGA GPFGDPMDQE DPGPPSEEEE LQRSVTAHNR LQQLRSTTDL LPKRPQHLPG HPGWYRQLCP RLDDPAWAPG PSLLSLQMRV TPKLMALTWD GFPLHYSDSH GWGYLVPGRR DNLTEPPVSP TVESAAVTCP YRAIESLYRK HCLEQGKQQL EPQEVDLAEE FLLTDNSAMW QTVEELGCLD VEAEAKMENS GLSQPLVLPA ACAPKSSQPT YHHGNGPYND VNIPGCWFFK LPHKDGNNYN VGSPFAKDFL PKMEDGTLQA GPGGASGPRA LEINKMISFW RNAHKRISSQ MVVWLPRSAL PRVVTRHPAF DEEGHYGAIL PQVVTAGTIT RRAVEPTWLT ASNARPDRVG SELKAMVQAP PGYVLVGADV DSQELWIAAV LGDAHFAGMH GCTAFGWMTL QGRKSRGTDL HSKTAATVGI SREHAKIFNY GRIYGAGQSF AERLLMQFNH RLTRQEAAEK AQQMYAVTKG LRRYRLSADG EWLVKQLNLP VDRTEDGWVS LQDLRMIRRE ASRKSRWKKW EVAAERAWTG GTESEMFNKL ESIAMSDTPR TPVLGCCISR ALEPSVVQGE FITSRVNWVV QSSAVDYLHL MLVAMKWLFE EFAIDGRFCI SIHDEVRYLV REEDRYRAAL ALQITNLLTR CMFAYKLGLN DLPQSVAFFS AVDIDQCLRK EVTMDCKTPS NPTGMERRYG IPQGEALDIY QIIELTKGSL EKRSQPGP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product POLG 蛋白
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- POLG (Polymerase (DNA Directed), gamma (POLG))
- 别名
- Polg (POLG 产品)
- 别名
- POLG Protein, MDP1 Protein, MIRAS Protein, MTDPS4A Protein, MTDPS4B Protein, PEO Protein, POLG1 Protein, POLGA Protein, SANDO Protein, SCAE Protein, AA409516 Protein, PolgA Protein, polymerase (DNA) gamma, catalytic subunit L homeolog Protein, DNA polymerase gamma, catalytic subunit Protein, polymerase (DNA directed), gamma Protein, polg.L Protein, Polg Protein, POLG Protein, polg Protein
- 物质类
- Viral Protein
- 背景
- DNA polymerase subunit gamma-1 (EC 2.7.7.7) (3'-5' exodeoxyribonuclease) (EC 3.1.11.-) (5'-deoxyribose-phosphate lyase) (EC 4.2.99.-) (Mitochondrial DNA polymerase catalytic subunit) (PolG-alpha) (PolgA),FUNCTION: Catalytic subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates (PubMed:26095671) (By similarity). Has 5' -> 3' polymerase activity. Functionally interacts with TWNK and SSBP1 at the replication fork to form a highly processive replisome, where TWNK unwinds the double-stranded DNA template prior to replication and SSBP1 covers the parental heavy strand to enable continuous replication of the entire mitochondrial genome. A single nucleotide incorporation cycle includes binding of the incoming nucleotide at the insertion site, a phosphodiester bond formation reaction that extends the 3'-end of the primer DNA, and translocation of the primer terminus to the post-insertion site. After completing replication of a mtDNA strand, mediates 3' -> 5' exonucleolytic degradation at the nick to enable proper ligation (PubMed:26095671) (By similarity). Highly accurate due to high nucleotide selectivity and 3' -> 5' exonucleolytic proofreading. Proficiently corrects base substitutions, single-base additions and deletions in non-repetitive sequences and short repeats, but displays lower proofreading activity when replicating longer homopolymeric stretches. Exerts exonuclease activity toward single-stranded DNA and double-stranded DNA containing 3'-terminal mispairs. When a misincorporation occurs, transitions from replication to a pro-nucleolytic editing mode and removes the missincorporated nucleoside in the exonuclease active site. Proceeds via an SN2 nucleolytic mechanism in which Asp-198 catalyzes phosphodiester bond hydrolysis and Glu-200 stabilizes the leaving group. As a result the primer strand becomes one nucleotide shorter and is positioned in the post-insertion site, ready to resume DNA synthesis (PubMed:15164064, PubMed:26095671) (By similarity). Exerts 5'-deoxyribose phosphate (dRP) lyase activity and mediates repair-associated mtDNA synthesis (gap filling) in base-excision repair pathway. Catalyzes the release of the 5'-terminal 2-deoxyribose-5-phosphate sugar moiety from incised apurinic/apyrimidinic (AP) sites to produce a substrate for DNA ligase. The dRP lyase reaction does not require divalent metal ions and likely proceeds via a Schiff base intermediate in a beta-elimination reaction mechanism (By similarity). {ECO:0000250|UniProtKB:P54098, ECO:0000269|PubMed:15164064, ECO:0000269|PubMed:26095671}.
- 分子量
- 136.8 kDa
- UniProt
- P54099
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