BRCAA1 Protein (AA 1-1314) (Strep Tag)
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- 抗原 See all BRCAA1 (ARID4B) products
- BRCAA1 (ARID4B) (RBP1-like protein (ARID4B))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1314
- 宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This BRCAA1 protein is labelled with Strep Tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
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MKALDEPPYL TVGTDVSAKY RGAFCEAKIK TAKRLVKVKV TFRHDSSTVE VQDDHIKGPL KVGAIVEVKN LDGAYQEAVI NKLTDASWYT VVFDDGDEKT LRRSSLCLKG ERHFAESETL DQLPLTNPEH FGTPVIGKKT NRGRRSNHIP EEESSSSSSD DDEEERKQTD ELLGKVVCVD YVSLEKKKAM WFPALVVCPD CSDEIAVKKD NILVRSFKDG KFTSVPRKDV HEITSDTVPK PDAVLKQAFD QALEFHKSRA IPANWKTELK EDSSSSEAEE EEEEEDDEKE KEDNSSEEEE EIEPFPEERE NFLQQLYKFM EDRGTPINKR PVLGYRNLNL FKLFRLVHKL GGFDNIESGA VWKQVYQDLG IPVLNSAAGY NVKCAYKKYL YGFEEYCRSA NIDFQMALPE KVLNKPCKDC ENKEVKVKEE SETEIKEVNV EDSKNVMPKE ETPAEDESER KENIKPSLGS KKSLLECIPA QSDEEKEAHI TKLEENENLE DKDGGRARTE EAFSTEVDGE EEQARSGDET NKEEDEDDEE IEEEEEEDEE EDEDEDDDDN NEEEEFECYP PGMKVQVRYG RGKNQKMYEA SIKDSDVEGG EALYLVHYCG WNVRYDEWIK ADKIVRPADK NVPKIKHRKK IKNKLDKEKD RDEKYSPKNC KLRRLSKSPF QSNPSPEMVS KLDLADAKNS DTAHIKSIEI TSILNGLQAS ESSAEDSEQE DERCTQDVDN IGKDESKVEH STHSRNELIS KEEQSSPSLL EENKVHTDLV IAKTVSKSPE RLRKDMEAIS EDTDFEEEDE ITKKRKDVKK DTTDKALKPQ TKRGKRRYCS ADECLQTGSP GKKEDRTKSK EPLCTENSSN SSSDEDEEEK SKAKMTPTKK YNGLEEKRKS LRTTSFYSGF SEVAEKRIKL LNNSDERLQN NRAKDRKDVW SSIQGQWPKK TLKELFSDSD TEAAASPPHP APDEGAVEES LQTVAEEESC SPIMELEKPL PASVDNKPIE EKPLEVSDRK TEFPSSGSNS VLNTPPTTPE SPSSVTITEA SQQQSSVTVS VPLPPNQEEV RSIKSETDST IEVDSVVGEL QDLQSEGNSS PAGFDASVSS SSSNQPEPDN PEKACTGQKR VKDTQGVGSS SKKQKRSHKA TVVNNKKKGK GTNSSDSEEL SAGESVTKTQ TIKSVPTGMK THNSKSPARV QSPGKGGRNG DKDPDLKEPS NRLPKVYKWS FQTSDLENMT SAERISILQE KLQEIRKHYL SLKSEVASID RRRKRLKKKE RESAATSSSS SSPSSSSITA AVMLTLAEPS MSSASQNGMS VECR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- BRCAA1 (ARID4B) (RBP1-like protein (ARID4B))
- 别名
- Arid4b (ARID4B 产品)
- 别名
- Bcaa Protein, BCAA Protein, BRCAA1 Protein, RBBP1L1 Protein, RBP1L1 Protein, SAP180 Protein, 5930400I17 Protein, 6330417L24Rik Protein, 6720480E17Rik Protein, 9330186M13 Protein, Rbp1l1 Protein, AT-rich interaction domain 4B Protein, AT rich interactive domain 4B (RBP1-like) Protein, Arid4b Protein, ARID4B Protein
- 背景
- AT-rich interactive domain-containing protein 4B (ARID domain-containing protein 4B) (180 kDa Sin3-associated polypeptide) (Sin3-associated polypeptide p180) (Histone deacetylase complex subunit SAP180),FUNCTION: Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (By similarity). Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A (PubMed:17043311). Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR (androgen receptor) and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier (PubMed:23487765). Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor (PubMed:18728284). {ECO:0000250|UniProtKB:Q4LE39, ECO:0000269|PubMed:17043311, ECO:0000269|PubMed:18728284, ECO:0000269|PubMed:23487765}.
- 分子量
- 147.6 kDa
- UniProt
- A2CG63
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