PLCH2 Protein (AA 1-1501) (Strep Tag)
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- 抗原 See all PLCH2 (PLCh2) products
- PLCH2 (PLCh2) (Phospholipase C eta 2 (PLCh2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1501
- 宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This PLCH2 protein is labelled with Strep Tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- 序列
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MGGLAWGPSR AAGSSWVNAS GTWEQPLRGF SGLQGGRRRG RGEKGIPEEP LCQLTPQLGL SLRVPFGLGD YGLDMPGPQP SAASQTTGAV ACLAEVLLWV GGSVVVSPRW QLSLVVERCM SAMQEGTQMV KLRGSSKGLV RFYYLDEHRS CLRWRPSRKN EKAKISIDSI QEVSEGRQSE IFQRYPDSSF DPNCCFSIYH GSHRESLDLV SPSSEEARTW VTGLRYLMAG ISDEDSLARR QRTRDQWLKQ TFDEADKNGD GSLSISEVLQ LLHKLNVNLP RQRVKQMFRE ADTDDHQGTL GFEEFCAFYK MMSTRRDLYL LMLTYSNHKD HLDASDLQRF LEVEQKMNGV TLESCQNIIE QFEPCLENKS KGMLGIDGFT NYTRSPAGDI FNPEHNRVHQ DMTQPLSHYF ITSSHNTYLV GDQLMSQSRV DMYAWVLQAG CRCVEVDCWD GPDGEPIVHH GYTLTSKILF KDVIETINKY AFIKNEYPVI LSIENHCSVV QQKKMAQYLT DILGDKLDLS SVSSEDATML PSPQMLKGKI LVKGKKLPAN ISEDAEEGEV SDEDSADEME DDCKLLNGDA STNRKRVENI AKKKLDSLIK ESKIRDCEDP NDFSVSTLSP SGKLGRKAEA KKGQSKVEED VEAGEDSGVS RQNSRLFMSS FSKRKKKGSK IKKVASVEEG DETLDSPGSQ SRGTARQKKT MKLSRALSDL VKYTKSVGTH DVEIEVVSSW QVSSFSETKA HQILQQKPTQ YLRFNQHQLS RIYPSSYRVD SSNYNPQPFW NAGCQMVALN YQSEGRMLQL NRAKFSANGD CGYVLKPQCM CQGVFNPNSE DPLPGQLKKQ LALRIISGQQ LPKPRDSVLG DRGEIIDPFV EVEVIGLPVD CSKEQTRVVD DNGFNPMWEE TLVFTVHMPE IALVRFLVWD HDPIGRDFIG QRTLAFSSIM PGYRHVYLEG MEEASIFVHV AVSDISGKVK QTLGLKGLFL RGTKPGSLDS HAAGQPLPRP SVSQRLLRRT ASAPTKSQKP SRKGFPELAL GTQDAGSEGA ADDVAPSSPN PALEAPTQER SGSSSPRDTR LFPLQRPISP LCSLEPIAEE PALGPGLPLQ AAAPTGPSQE GSQCPVGLGA KVTSSQQTSL GAFGTLQLRI GGGRENEEPP LRPHNGGISS GPREGTSGRQ TDSKSRSRVP GHLPVVRRAK SEGQVLSELS PTPAVYSDAT GTDRLWQRLE PGSHRDSVSS SSSMSSNDTV IDLSLPSLGL CRSRESIPGV SLGRLTSRPC LASAARPDLP PVTKSKSNPN LRVAGGLPTA PDELQPRPLA PRLTGHHPRP PWHHLTLVGL RDCPVSAKSK SLGDLTADDF APSFQGSTSS LSCGLGSLGV AHQVLEPGIR RDALTEQLRW LTGFQQAGDI TSPTSLGPAG DGSVGGPSFL RRSSSRSQSR VRAIASRARQ AQERQQRLRG QDSRGPPEEE RGTPEGACSV GHEGCVDVPM PAKGAPEQVC GAADGQLLLR L
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- PLCH2 (PLCh2) (Phospholipase C eta 2 (PLCh2))
- 别名
- Plch2 (PLCh2 产品)
- 别名
- PLC-eta2 Protein, PLCL4 Protein, PLCeta2 Protein, A930027K05Rik Protein, Plc-eta2 Protein, Plcl4 Protein, RGD1304675 Protein, phospholipase C eta 2 Protein, phospholipase C, eta 2 Protein, PLCH2 Protein, Plch2 Protein
- 背景
- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 (EC 3.1.4.11) (Phosphoinositide phospholipase C-eta-2) (Phosphoinositide phospholipase C-like 4) (PLC-L4) (Phospholipase C-like protein 4) (Phospholipase C-eta-2) (PLC-eta2),FUNCTION: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This phospholipase activity is very sensitive to calcium. May be important for formation and maintenance of the neuronal network in the postnatal brain. {ECO:0000269|PubMed:15899900, ECO:0000269|PubMed:16107206}.
- 分子量
- 164.3 kDa
- UniProt
- A2AP18
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