APPL2 Protein (AA 1-662) (Strep Tag)
-
- 抗原 See all APPL2 蛋白
- APPL2 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 2 (APPL2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-662
- 宿主
- 小鼠
-
资源
- Tobacco (Nicotiana tabacum)
- 标记
- This APPL2 protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
-
MPAVDKLLLE EALQDSPQAR SLLSVFEEDA GTLTDYTNQL LQAMQRVYGA QNEMCLATQQ LSRQLLAYEK QNFALGKGDE EVISTLHYFS KVMDELNGLH TELAKQLADT MVLPVIQFRE KDLTEVSTLK DLFGLASSEH DLSMAKYSRL PKKKENEKAK TEIVKEVAAA RRKQHLSSLQ YYCALNALQY RKRAAMMEPL IGFAHGQINF FKRGAEMFSK SMDGFLSSVK DMVQSIQVEL EAEADKMRVS QQELLSVSES VYTPDIDVAT AQINRNLIQK TGYLNLRNKT GLVTTTWERL YFFTQGGNLM CQPRGAVAGG LIQDLDNCSV MAVDCEDRRY CFQISTPSGK PGIILQAESR KEYEEWICAV NNISRQIYLT DNPEAVAIKL NQTALQAVTP ITSFGKKQES SCSSQNIKNS DIEDDNIVPK ATASIPETEE LIAPGTPIQF DIVLPATEFL DQNRGGRRTN PFGETEDGSF PEAEDSLLQQ MFIVRFLGSM AVKTDSTAEV IYEAMRQVLA ARAIHNIFRM TESHLMVTSQ TLRLIDPQTQ VSRACFELTS VTQFAAHQEN KRLVGFVIRV PESTGEESLS TYIFESNSEG EKICYAINLG KEIIEVQKDP EALARLMLSV PLTNDGKYVL LNDQADDTGG SPSENRGAES EA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product APPL2 蛋白
-
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- APPL2 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 2 (APPL2))
- 别名
- Appl2 (APPL2 产品)
- 别名
- RGD1563028 Protein, MGC84621 Protein, dip13b Protein, APPL2 Protein, DIP13B Protein, Dip3b Protein, adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 Protein, adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 L homeolog Protein, adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 Protein, Appl2 Protein, APPL2 Protein, appl2.L Protein, appl2 Protein
- 背景
- DCC-interacting protein 13-beta (Dip13-beta) (Adapter protein containing PH domain, PTB domain and leucine zipper motif 2),FUNCTION: Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism (PubMed:25568335, PubMed:27219021, PubMed:25328665, PubMed:19661063, PubMed:29467283). Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex (By similarity). Plays a role in immune response by modulating phagocytosis, inflammatory and innate immune responses (PubMed:25568335, PubMed:27219021, PubMed:25328665). In macrophages, enhances Fc-gamma receptor-mediated phagocytosis through interaction with RAB31 leading to activation of PI3K/Akt signaling (PubMed:25568335). In response to LPS, modulates inflammatory responses by playing a key role on the regulation of TLR4 signaling and in the nuclear translocation of RELA/NF-kappa-B p65 and the secretion of pro- and anti-inflammatory cytokines (PubMed:27219021). Also functions as a negative regulator of innate immune response via inhibition of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1 (PubMed:25328665). Plays a role in endosomal trafficking of TGFBR1 from the endosomes to the nucleus (By similarity). Plays a role in cell metabolism by regulating adiponectin and insulin signaling pathways and adaptative thermogenesis (PubMed:19661063, PubMed:29467283) (By similarity). In muscle, negatively regulates adiponectin-simulated glucose uptake and fatty acid oxidation by inhibiting adiponectin signaling pathway through APPL1 sequestration thereby antagonizing APPL1 action (PubMed:19661063). In muscles, negativeliy regulates insulin-induced plasma membrane recruitment of GLUT4 and glucose uptake through interaction with TBC1D1 (By similarity). Plays a role in cold and diet-induced adaptive thermogenesis by activating ventromedial hypothalamus (VMH) neurons throught AMPK inhibition which enhances sympathetic outflow to subcutaneous white adipose tissue (sWAT), sWAT beiging and cold tolerance (PubMed:29467283). Also plays a role in other signaling pathways namely Wnt/beta-catenin, HGF and glucocorticoid receptor signaling (PubMed:28965332, PubMed:29675572, PubMed:26445298). Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex (By similarity). May affect adult neurogenesis in hippocampus and olfactory system via regulating the sensitivity of glucocorticoid receptor (PubMed:28965332, PubMed:29675572). Required for fibroblast migration through HGF cell signaling (PubMed:26445298). {ECO:0000250|UniProtKB:Q8NEU8, ECO:0000269|PubMed:19661063, ECO:0000269|PubMed:25328665, ECO:0000269|PubMed:25568335, ECO:0000269|PubMed:26445298, ECO:0000269|PubMed:27219021, ECO:0000269|PubMed:28965332, ECO:0000269|PubMed:29467283, ECO:0000269|PubMed:29675572}.
- 分子量
- 73.9 kDa
- UniProt
- Q8K3G9
-