HBS1L Protein (AA 1-682) (Strep Tag)
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- 抗原 See all HBS1L 蛋白
- HBS1L (HBS1-Like (HBS1L))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-682
- 宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This HBS1L protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MARHRNVRGY NYDEDFEDDD LYGQSVEDDY CISPSTAAQF IYSRRDNPEE EYGYEDLRES SNSLLNHQLS EIDQARLYSC LDHMREVLGD AVPDDILTEA ILKHKFDVQK ALSVVLEQDG VQPWKEKSER AVCAGQPSKG KSVISRSSQS ESEIVPKVAK MTVSGKKQTM GFEVPGLTSE ENGLSVRAPH KGPPGDDVSV ASPNIPETGT PKSALPPPSL QTSEELGSTP TPVRKSGKLR QQIDVKAELE KRQGGKQLLN LVVIGHVDAG KSTLMGHMLY LLGNVNKRTM HKYEQESKKA GKASFAYAWV LDETGEERER GVTMDVGMTK FETTTKVITL MDAPGHKDFI PNMITGAAQA DVAVLVVDAS RGEFEAGFET GGQTREHGLL VRSLGVTQLA VAVNKMDQVN WQQERFQEIT GKLGHFLKQA GFKESDVAFI PTSGLSGENL TARSQSSDLT TWYKGMCLLE QIDSFKPPQR SIDKPFRLCV SDVFKDQGSG FCVTGKIEAG YIQTGDRLLA MPPNETCTAK GITLHDEPVD WAAAGDHVNL TLVGMDIIKI NVGCIFCGPK EPIKACTRFR ARILVFNIEV PITKGFPVLL HYQTVSEPAV IKRLISVLNK STGEVTKKKP KLLTKGQNAL VELQTQRPVA LELYKDFKEL GRFMLRYGGS TVAAGVVTEI KE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product HBS1L 蛋白
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- HBS1L (HBS1-Like (HBS1L))
- 别名
- Hbs1l (HBS1L 产品)
- 别名
- EF-1a Protein, ERFS Protein, HBS1 Protein, HSPC276 Protein, 2810035F15Rik Protein, AI326327 Protein, eRFS Protein, wu:fc23c07 Protein, zgc:55400 Protein, EFL1-alpha Protein, chunp6927 Protein, eef1a Protein, ef1a Protein, ik:tdsubc_2a3 Protein, ik:tdsubc_2b3 Protein, tdsubc_2a3 Protein, wu:fa91c07 Protein, wu:fa94b03 Protein, wu:fi13b09 Protein, xx:tdsubc_2a3 Protein, xx:tdsubc_2b3 Protein, Hbs1l Protein, HBS1 like translational GTPase Protein, Hbs1-like (S. cerevisiae) Protein, HBS1-like translational GTPase Protein, elongation factor 1 alpha like protein Protein, HBS1-like (S. cerevisiae) Protein, eukaryotic translation elongation factor 1 alpha 1, like 1 Protein, HBS1 like translational GTPase L homeolog Protein, HBS1-like protein Protein, HBS1L Protein, Hbs1l Protein, hbs1l Protein, SJAG_02601 Protein, eef1a1l1 Protein, hbs1l.L Protein, LOC101787962 Protein
- 物质类
- Viral Protein
- 背景
- HBS1-like protein (EC 3.6.5.-),FUNCTION: GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway. {ECO:0000250|UniProtKB:Q9Y450}.
- 分子量
- 75.1 kDa
- UniProt
- Q69ZS7
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