QRICH1 Protein (AA 1-777) (Strep Tag)
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- 抗原 See all QRICH1 products
- QRICH1 (Glutamine-Rich 1 (QRICH1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-777
- 宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This QRICH1 protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MNNSLENTIS FEEYIRVKAR SVPQHRMKEF LDSLASKGPE ALQEFQQTAT TTMVYQQGGN CIYTDSTEVA GSLLELACPV TTSVQPQTQQ EQQIQVQQPQ QVQVQVQVQQ SPQQVSAQQL SPQFTVHQPA EQPIQVQVQI QGQAPQSAAP SIQTPSLQSP SPSQLQAAQI QVQHVQAAQQ IQAAEIPEEH IPHQQIQAQL VAGQSLAGGQ QIQIQTVGAL SPPPSQQGSP REGERRVGTA SVLQPVKKRK VDMPITVSYA ISGQPVATVL AIPQGQQQSY VSLRPDLLTV DSAHLYSATG TITSPTGETW TIPVYSAQPR GDPQQQSITH IAIPQEAYNA VHVSGSPKAL AAVKLEDDKE KMVGTTSVVK NSHEEVVQTL ANSLFPAQFM NGNIHIPVAV QAVAGTYQNT AQTVHIWDPQ QQPQQQTAQE QTPPPQQQQQ QLQVTCSAQT VQVAEVEPQS QPQPSPELLL PNSLKPEEGL EVWKNWAQTK NAELEKDAQN RLAPIGRRQL LRFQEDLISS AVAELNYGLC LMTREARNGE GEPYDPDVLY YIFLCIQKYL FENGRVDDIF SDLYYVRFTE WLHEVLKDVQ PRVTPLGYVL PSHVTEEMLW ECKQLGAHSP STLLTTLMFF NTKYFLLKTV DQHMKLAFSK VLRQTKKSPS NPKDKSTSIR YLKALGIHQT GQKVTDDMYA EQTENPENPL RCPIKLYDFY LFKCPQSVKG RNDTFYLTPE PVVAPNSPIW YSVQPISREQ MGQMLTRILV IREIQEAIAV ANATTMH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- QRICH1 (Glutamine-Rich 1 (QRICH1))
- 别名
- Qrich1 (QRICH1 产品)
- 别名
- fj59h09 Protein, MGC110077 Protein, wu:fj59h09 Protein, MGC81284 Protein, MGC89757 Protein, 2610028H07Rik Protein, glutamine-rich 1 Protein, glutamine-rich 1, gene 1 L homeolog Protein, glutaminyl-tRNA synthetase Protein, glutamine rich 1 Protein, glutamine-rich 1, gene 1 Protein, qrich1 Protein, qrich1.1.L Protein, QARS Protein, QRICH1 Protein, qrich1.1 Protein, Qrich1 Protein
- 背景
- Transcriptional regulator QRICH1 (Glutamine-rich protein 1),FUNCTION: Transcriptional regulator that acts as a mediator of the integrated stress response (ISR) through transcriptional control of protein homeostasis under conditions of ER stress (PubMed:33384352). Controls the outcome of the unfolded protein response (UPR), an ER-stress response pathway that either promotes recovery of ER homeostasis and cell survival, or triggers the terminal UPR which elicits programmed cell death when ER stress is prolonged and unresolved (PubMed:33384352). ER stress induces QRICH1 translation by a ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced QRICH1 regulates a transcriptional program associated with protein translation, protein secretion-mediated proteotoxicity and cell death during the terminal UPR (By similarity). May cooperate with ATF4 transcription factor signaling to regulate ER homeostasis which is critical for cell viability (By similarity). Up-regulates CASP3/caspase-3 activity in epithelial cells under ER stress. Central regulator of proteotoxicity associated with ER stress-mediated inflammatory diseases in the intestines and liver (PubMed:33384352). Involved in chondrocyte hypertrophy, a process required for normal longitudinal bone growth (PubMed:30281152). {ECO:0000250|UniProtKB:Q2TAL8, ECO:0000269|PubMed:30281152, ECO:0000269|PubMed:33384352}.
- 分子量
- 86.6 kDa
- UniProt
- Q3UA37
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