FEZF1 Protein (AA 1-475) (Strep Tag)
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- 抗原 See all FEZF1 蛋白
- FEZF1 (FEZ Family Zinc Finger 1 (FEZF1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-475
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宿主
- 人
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This FEZF1 protein is labelled with Strep Tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
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MDSSCHNATT KMLATAPARG NMMSTSKPLA FSIERIMART PEPKALPVPH FLQGALPKGE PKHSLHLNSS IPCMIPFVPV AYDTSPKAGV TGSEPRKASL EAPAAPAAVP SAPAFSCSDL LNCALSLKGD LARDALPLQQ YKLVRPRVVN HSSFHAMGAL CYLNRGDGPC HPAAGVNIHP VASYFLSSPL HPQPKTYLAE RNKLVVPAVE KYPSGVAFKD LSQAQLQHYM KESAQLLSEK IAFKTSDFSR GSPNAKPKVF TCEVCGKVFN AHYNLTRHMP VHTGARPFVC KVCGKGFRQA STLCRHKIIH TQEKPHKCNQ CGKAFNRSST LNTHTRIHAG YKPFVCEFCG KGFHQKGNYK NHKLTHSGEK QFKCNICNKA FHQVYNLTFH MHTHNDKKPF TCPTCGKGFC RNFDLKKHVR KLHDSSLGLA RTPAGEPGTE PPPPLPQQPP MTLPPLQPPL PTPGPLQPGL HQGHQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product FEZF1 蛋白
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- FEZF1 (FEZ Family Zinc Finger 1 (FEZF1))
- 别名
- FEZF1 (FEZF1 产品)
- 别名
- RGD1560480 Protein, fezf Protein, znf312b Protein, MGC145310 Protein, FEZ Protein, ZNF312B Protein, 3110069A13Rik Protein, Fez Protein, Zfp312-like Protein, fez-like Protein, fez Protein, fezf2 Protein, wu:fc29c05 Protein, wu:fc89h05 Protein, zgc:153730 Protein, Fez family zinc finger 1 Protein, FEZ family zinc finger 1 Protein, Fez family zinc finger protein 1 Protein, FEZ family zinc finger 1 S homeolog Protein, Fezf1 Protein, FEZF1 Protein, fezf1 Protein, Tsp_12536a Protein, Tsp_12536 Protein, fezf1.S Protein
- 背景
- Fez family zinc finger protein 1 (Zinc finger protein 312B),FUNCTION: Transcription repressor. Involved in the axonal projection and proper termination of olfactory sensory neurons (OSN). Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation. Expression is required in OSN to cell-autonomously regulate OSN axon projections. Regulates non-cell-autonomously the layer formation of the olfactory bulb development and the interneurons. May be required for correct rostral migration of the interneuron progenitors (By similarity). {ECO:0000250}.
- 分子量
- 52.0 kDa
- UniProt
- A0PJY2
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