NAD Synthetase (Active) 蛋白
宿主: Bacillus subtilis
宿主: 大肠杆菌（E. Coli）
Recombinant
>98 % (SDS-PAGE)
SDS
Active
-
- 抗原
- NAD Synthetase
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 宿主
- Bacillus subtilis
-
资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 产品特性
- Full-length NAD synthetase (Bacillus subtilis).
- 纯度
- >98 % (SDS-PAGE)
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
~0.3 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol NAD per min.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Liquid. In 50 mM TRIS-HCl, pH 8, containing 75 mM sodium chloride, 5 % glycerol and 5 mM DTT.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,-80 °C
- 储存方法
-
Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 6 months after receipt when stored at -80°C. - 有效期
- 6 months
-
- 抗原
- NAD Synthetase
- 背景
- NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It was shown to be induced by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation and is involved in biological processes like stress response and sporulation resulting in formation of a cellular spore. Catalytic activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+
- 分子量
- ~30.4kDa
- UniProt
- P08164
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