WISP1 Protein (Transcript Variant 1)
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- 抗原 See all WISP1 蛋白
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human WISP1 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 ug/ml.
- Top Product
- Discover our top product WISP1 蛋白
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WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 23-367) protein (His tag)
WISP1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 1-280) protein (GST tag)WISP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) proteinWISP1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)WISP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
- 别名
- Wisp1 (WISP1 产品)
- 别名
- CCN4 Protein, WISP1c Protein, WISP1i Protein, WISP1tc Protein, WISP-1 Protein, WISP1 Protein, si:ch211-38f10.2 Protein, AW146261 Protein, Elm1 Protein, zgc:172276 Protein, WNT1 inducible signaling pathway protein 1 Protein, WNT1 inducible signaling pathway protein 1a Protein, WNT1-inducible-signaling pathway protein 1-like Protein, WNT1 inducible signaling pathway protein 1b Protein, WISP1 Protein, wisp1a Protein, Wisp1 Protein, LOC100627044 Protein, wisp1b Protein
- 背景
- This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83 % identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.
- 分子量
- 38.1 kDa
- NCBI登录号
- NP_003873
- 途径
- WNT signaling, Growth Factor Binding
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