VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))
蛋白类型
Recombinant
生物活性
Active
产品特性
Transcript Variant 1
宿主
小鼠
资源
大肠杆菌(E. Coli)
应用范围
Antibody Production (AbP), Functional Studies (Func), Standard (STD), Protein Interaction (PI)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human VEGF-A (transcript variant 1) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
内毒素水平
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-5.0 ng/ml.
VEGFA
宿主: 小鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % as determined by SDS-PAGE
WB, ELISA
应用备注
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
缓冲液
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
注意事项
Resuspend the protein in the desired concentration in proper buffer
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
抗原
VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))