Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human Tumor necrosis factor (TNF-alpha) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
Purity is > 98 % , as determined by Coomassie stained SDS-PAGE.
内毒素水平
Less than 0.01 ng per ug protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 0.020-0.050 ng/ml, corresponding to a specific activity of 5-2 X10^7 units/mg, determined by a dose dependent stimulation of L929 cells treated with actinomycin D.
TNF alpha
宿主: 犬
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % by SDS-PAGE gel analyses
WB, ELISA
应用备注
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
浓度
> 50 μg/mL
缓冲液
10 mM NaH2PO4, 150 mM NaCl, pH 7.2
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.