Quick Overview for SLIT2 蛋白 (ABIN2732203)
抗原
See all SLIT2 蛋白
SLIT2
(Slit Homolog 2 (Drosophila) (SLIT2))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
HEK-293 Cells
应用范围
Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human SLIT2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Determined by its ability to inhibit MC3T3/E1 osteoblasts cell differentiation.
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))
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别名
- Slit2
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背景
- This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants.
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分子量
- 120 kDa
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NCBI登录号
- NP_004778
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途径
- Regulation of Actin Filament Polymerization, Regulation of Cell Size, Smooth Muscle Cell Migration
抗原
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