Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human SERPINE1 / PAI1 (transcript variant 1) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
内毒素水平
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37C. Half maximal inhibition against 1.0 ug/mL of single chain tPA was obtained at a concentration of 2.0ug/mL.
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
缓冲液
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
注意事项
Resuspend the protein in the desired concentration in proper buffer
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
PAI Protein, PAI-1 Protein, PAI1 Protein, PLANH1 Protein, SERPINE1 Protein, si:ch211-138a11.1 Protein, Planh1 Protein, PAI1A Protein, Pai1 Protein, Pai1aa Protein, Planh Protein, RATPAI1A Protein, serpin family E member 1 Protein, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog Protein, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 Protein, serine (or cysteine) peptidase inhibitor, clade E, member 1 Protein, SERPINE1 Protein, serpine1.L Protein, serpine1 Protein, Serpine1 Protein
背景
This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.