NF-kB p65 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- 抗原 See all NF-kB p65 (NFkBP65) 蛋白
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This NF-kB p65 protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human RELA / NF-kB p65 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- RELA Activity Verified in a DNA-binding Assay: RELA activity was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the RELA consensus DNA-binding sequence was incubated with dilutions of the purified RELA protein. RELA bound to the oligo was captured onto the surface of a microtiter plate and after washing, bound RELA was detected with an anti-RELA primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm.
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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The M. tuberculosis HAD phosphatase (Rv3042c) interacts with host proteins and is inhibited by Clofazimine." in: Cellular and molecular life sciences : CMLS, Vol. 73, Issue 17, pp. 3401-17, (2016) (PubMed).
: "Functional characterization of a competitive peptide antagonist of p65 in human macrophage-like cells suggests therapeutic potential for chronic inflammation." in: Drug design, development and therapy, Vol. 8, pp. 2409-21, (2015) (PubMed).
: "MYB elongation is regulated by the nucleic acid binding of NFκB p50 to the intronic stem-loop region." in: PLoS ONE, Vol. 10, Issue 4, pp. e0122919, (2015) (PubMed).
: "FOXO3-NF-κB RelA Protein Complexes Reduce Proinflammatory Cell Signaling and Function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 12, pp. 5637-47, (2015) (PubMed).
: "Protective role of PGC-1α in diabetic nephropathy is associated with the inhibition of ROS through mitochondrial dynamic remodeling." in: PLoS ONE, Vol. 10, Issue 4, pp. e0125176, (2015) (PubMed).
: "MFN2 couples glutamate excitotoxicity and mitochondrial dysfunction in motor neurons." in: The Journal of biological chemistry, Vol. 290, Issue 1, pp. 168-82, (2015) (PubMed).
: "S6K is a morphogenic protein with a mechanism involving Filamin-A phosphorylation and phosphatidic acid binding." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 4, pp. 1299-313, (2015) (PubMed).
: "ATM-mediated PTEN phosphorylation promotes PTEN nuclear translocation and autophagy in response to DNA-damaging agents in cancer cells." in: Autophagy, Vol. 11, Issue 2, pp. 239-52, (2015) (PubMed).
: "Heterogeneous Nuclear Ribonucleoprotein M Facilitates Enterovirus Infection." in: Journal of virology, Vol. 89, Issue 14, pp. 7064-78, (2015) (PubMed).
: "Calponin-h2: a potential serum marker for the early detection of human breast cancer?" in: Tumour biology, Vol. 35, Issue 11, pp. 11121-7, (2014) (PubMed).
: "Thiamet-G-mediated inhibition of O-GlcNAcase sensitizes human leukemia cells to microtubule-stabilizing agent paclitaxel." in: Biochemical and biophysical research communications, Vol. 453, Issue 3, pp. 392-7, (2014) (PubMed).
: "Muscleblind-like 1 activates insulin receptor exon 11 inclusion by enhancing U2AF65 binding and splicing of the upstream intron." in: Nucleic acids research, Vol. 42, Issue 3, pp. 1893-903, (2014) (PubMed).
: "Mutations in the Matrin 3 gene cause familial amyotrophic lateral sclerosis. ..." in: Nature neuroscience, Vol. 17, Issue 5, pp. 664-6, (2014) (PubMed).
: "Hyperglycemia and redox status regulate RUNX2 DNA-binding and an angiogenic phenotype in endothelial cells." in: Microvascular research, Vol. 97, pp. 55-64, (2014) (PubMed).
: "Hepatitis C virus subverts liver-specific miR-122 to protect the viral genome from exoribonuclease Xrn2." in: Cell host & microbe, Vol. 16, Issue 2, pp. 257-64, (2014) (PubMed).
: "ZNF750 interacts with KLF4 and RCOR1, KDM1A, and CTBP1/2 chromatin regulators to repress epidermal progenitor genes and induce differentiation genes." in: Genes & development, Vol. 28, Issue 18, pp. 2013-26, (2014) (PubMed).
: "Inhibition of triple-negative and Herceptin-resistant breast cancer cell proliferation and migration by Annexin A2 antibodies." in: British journal of cancer, Vol. 111, Issue 12, pp. 2328-41, (2014) (PubMed).
: "ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN." in: Human molecular genetics, Vol. 22, Issue 18, pp. 3690-704, (2013) (PubMed).
: "The E3 ubiquitin ligase mind bomb 1 ubiquitinates and promotes the degradation of survival of motor neuron protein." in: Molecular biology of the cell, Vol. 24, Issue 12, pp. 1863-71, (2013) (PubMed).
: "HER2 phosphorylates and destabilizes pro-apoptotic PUMA, leading to antagonized apoptosis in cancer cells." in: PLoS ONE, Vol. 8, Issue 11, pp. e78836, (2013) (PubMed).
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The M. tuberculosis HAD phosphatase (Rv3042c) interacts with host proteins and is inhibited by Clofazimine." in: Cellular and molecular life sciences : CMLS, Vol. 73, Issue 17, pp. 3401-17, (2016) (PubMed).
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- 抗原
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- 别名
- Rela,nf-Kb p65 (NFkBP65 产品)
- 别名
- C-Rel Protein, NFKB3 Protein, p65 Protein, NFkB Protein, c-rel Protein, zgc:100833 Protein, Xrel2 Protein, Xrel3 Protein, rel Protein, rel-A Protein, rel2 Protein, rel3 Protein, v-rel Protein, xrel Protein, REL proto-oncogene, NF-kB subunit Protein, RELA proto-oncogene, NF-kB subunit Protein, v-rel reticuloendotheliosis viral oncogene homolog A (avian) Protein, v-rel avian reticuloendotheliosis viral oncogene homolog Protein, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog Protein, REL Protein, RELA Protein, Rela Protein, Rel Protein, rel Protein, rel.L Protein
- 背景
- NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
- 分子量
- 60 kDa
- NCBI登录号
- NP_068810
- 途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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