PDGFA Protein (Transcript Variant 2)
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- 抗原 See all PDGFA 蛋白
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 2
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human PDGFA (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is less than or equal to 1 ng/ml, corresponding to a specific activity of > 1 x 10^6 units/mg.
- Top Product
- Discover our top product PDGFA 蛋白
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Platelet Derived Growth Factor A (PDGFA) (Active) protein
PDGFA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Platelet Derived Growth Factor A (PDGFA) (AA 1-211) protein (GST tag)PDGFA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Platelet Derived Growth Factor A (PDGFA) (AA 94-194) (Active) protein (His tag)PDGFA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Platelet Derived Growth Factor A (PDGFA) (AA 84-193) protein (GST tag)PDGFA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
- 别名
- Pdgfa (PDGFA 产品)
- 别名
- PDGF-A Protein, PDGF1 Protein, pdgfa Protein, pdgfa-2 Protein, zgc:100777 Protein, pdgf Protein, pdgf1 Protein, pdgf-a Protein, AA409063 Protein, CD142 Protein, Cf-3 Protein, Cf3 Protein, TF Protein, PDGFACP Protein, wu:fc32c12 Protein, zgc:153249 Protein, platelet derived growth factor subunit A Protein, platelet-derived growth factor alpha polypeptide a Protein, coagulation factor III Protein, platelet derived growth factor, alpha Protein, platelet derived growth factor subunit A S homeolog Protein, platelet-derived growth factor alpha polypeptide b Protein, PDGFA Protein, pdgfaa Protein, pdgfa Protein, F3 Protein, Pdgfa Protein, pdgfa.S Protein, pdgfab Protein
- 背景
- This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.
- 分子量
- 28.5 kDa
- NCBI登录号
- NP_002598
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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