Nucleolin Protein (NCL) (Myc-DYKDDDDK Tag)
(3 references)-
- 抗原 See all Nucleolin (NCL) 蛋白
- Nucleolin (NCL)
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Nucleolin protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human Nucleolin protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product NCL 蛋白
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Nucleolin (NCL) (AA 1-711) protein (His tag)
NCL 宿主: 猕猴 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Nucleolin (NCL) (AA 2-710) protein (His tag)NCL 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Nucleolin (NCL) (AA 2-707) protein (His tag)NCL 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Nucleolin (NCL) (AA 2-482), (partial) protein (His tag)NCL 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
Nucleolin (NCL) proteinNCL 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Nucleolin promotes in vitro translation of feline calicivirus genomic RNA." in: Virology, Vol. 489, pp. 51-62, (2016) (PubMed).
: "The G-quadruplex-forming aptamer AS1411 potently inhibits HIV-1 attachment to the host cell." in: International journal of antimicrobial agents, Vol. 47, Issue 4, pp. 311-6, (2016) (PubMed).
: "Nucleolin stabilizes G-quadruplex structures folded by the LTR promoter and silences HIV-1 viral transcription." in: Nucleic acids research, Vol. 43, Issue 18, pp. 8884-97, (2015) (PubMed).
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: "Nucleolin promotes in vitro translation of feline calicivirus genomic RNA." in: Virology, Vol. 489, pp. 51-62, (2016) (PubMed).
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- 抗原
- Nucleolin (NCL)
- 别名
- Nucleolin (NCL 产品)
- 别名
- C23 Protein, B530004O11Rik Protein, D0Nds28 Protein, D1Nds28 Protein, Nucl Protein, wu:fa12d03 Protein, zgc:152810 Protein, nucl Protein, LOC100282923 Protein, LOC100283058 Protein, nucleolin Protein, nucleolin S homeolog Protein, NCL Protein, Ncl Protein, ncl.S Protein, ncl Protein, LOC100195537 Protein, LOC100282923 Protein, LOC100283058 Protein
- 背景
- Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
- 分子量
- 76.4 kDa
- NCBI登录号
- NP_005372
- 途径
- Ribonucleoprotein Complex Subunit Organization
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