MIA Protein (Transcript Variant 1)
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- 抗原 See all MIA 蛋白
- MIA (Melanoma Inhibitory Activity (MIA))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human MIA (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6ug/mL.
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- Discover our top product MIA 蛋白
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Melanoma Inhibitory Activity (MIA) (Active) protein
MIA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Melanoma Inhibitory Activity (MIA) (AA 1-131) protein (GST tag)MIA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Melanoma Inhibitory Activity (MIA) protein (Myc-DYKDDDDK Tag)MIA 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- MIA (Melanoma Inhibitory Activity (MIA))
- 别名
- Mia (MIA 产品)
- 别名
- CD-RAP Protein, Mia1 Protein, Cdrap Protein, zgc:194157 Protein, mia Protein, CDRAP Protein, Fdp Protein, MIA Protein, MIAL Protein, melanoma inhibitory activity Protein, otoraplin Protein, cilia and flagella associated protein 100 Protein, MIA Protein, Mia Protein, mia Protein, Otor Protein, CFAP100 Protein
- 背景
- Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. [UniProtKB/Swiss-Prot Function]
- 分子量
- 12.2 kDa
- NCBI登录号
- NP_006524
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