MIA Protein (Transcript Variant 1)
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- 抗原 See all MIA 蛋白
- MIA (Melanoma Inhibitory Activity (MIA))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human MIA (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6ug/mL.
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- Discover our top product MIA 蛋白
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Melanoma Inhibitory Activity (MIA) (Active) protein
MIA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Melanoma Inhibitory Activity (MIA) (AA 1-131) protein (GST tag)MIA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Melanoma Inhibitory Activity (MIA) protein (Myc-DYKDDDDK Tag)MIA 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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