Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human Interleukin-33 (IL33) (transcript variant 1) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 98 % , as determined by Coomassie stained SDS-PAGE.
内毒素水平
Less than 0.01 ng per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 0.05 to 0.25 ng/ml, corresponding to a specific activity of 4 x 10^6 to 2 x 10^7 units/mg, determined by the dose dependent stimulation of anti-CD3 activated D10.G4.1 cell proliferation.
IL33
宿主: Cynomolgus
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
应用备注
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
浓度
> 50 μg/mL
缓冲液
1 x PBS
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene.