IL12A Protein (Transcript Variant 2)
IL12A
宿主: 小鼠
宿主: CHO Cells
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Func, AbP, STD, PI
Active
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- 抗原 See all IL12A 蛋白
- IL12A (Interleukin 12 alpha (IL12A))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 2
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宿主
- 小鼠
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资源
- CHO Cells
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human Interleukin-12 alpha / IL12A (transcript variant 2) protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Assay #1: ED50 was determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is > 0.1 ng/ml, corresponding to a specific activity of > 1 x 10^7 units/mg. Assay #2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is
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- Discover our top product IL12A 蛋白
-
Interleukin 12 alpha (IL12A) (AA 144-253) protein (GST tag)
IL12A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- IL12A (Interleukin 12 alpha (IL12A))
- 别名
- Interleukin-12 Alpha,il12a (IL12A 产品)
- 分子量
- 75 kDa
- NCBI登录号
- NP_032377
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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