p53 Protein (His tag)
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- 抗原 See all p53 (TP53) 蛋白
- p53 (TP53) (Tumor Protein P53 (TP53))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This p53 protein is labelled with His tag.
- 应用范围
- Standard (STD), Protein Interaction (PI), In vitro Assay (in vitro)
- 产品特性
- p53 is a tumor suppressor protein functioning as a sequence-specific transcription factor important for cell cycle arrest, apoptosis, and DNA-damage response. Recombinant p53, transcript variant 1, was expressed in E. coli from human cDNA (accession number NM_000546.5) with an N-terminal polyhistidine tag. Active Motif also offers Recombinant p53 protein (Catalog No. 31103) that was expressed in Baculovirus.
- Top Product
- Discover our top product TP53 蛋白
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Tumor Protein P53 (TP53) (AA 1-393) protein (His tag)
custom-made TP53 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Protein P53 (TP53) (AA 1-393) protein (GST tag)TP53 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tumor Protein P53 (TP53) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)TP53 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining STD, AbP
Tumor Protein P53 (TP53) (AA 1-391) protein (His tag)TP53 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Tumor Protein P53 (TP53) protein (His-SUMO Tag)TP53 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90% as determined by SDS-PAGE. ELISA
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- 应用备注
- Recombinant p53 is suitable for use in Active Motif's TransAM® assay as a protein standard, in vitro transcription assays, protein-protein interactions and protein-DNA interaction studies. A recommended starting point for in vitro transcription assays is 20-100 ng per reaction. The molecular weight of the protein is 53 kDa.
- 限制
- 仅限研究用
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- 注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 储存条件
- -80 °C
- 储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- 抗原
- p53 (TP53) (Tumor Protein P53 (TP53))
- 别名
- p53 (TP53 产品)
- 背景
- P53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis.
- 途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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