Recombinant JMJD2D / KDM4D (accession number NP_060509.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 63.2 kDa.
Recombinant JMJD2D / KDM4D is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant JMJD2D / KDM4D protein, and 3.3 μM H3K9me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
限制
仅限研究用
注意事项
Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
储存条件
-80 °C
储存方法
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
KDM4D (lysine (K)-specific demethylase 4D), also known as JMJD2D (Jumonji Domain Containing 2D) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 residues of histone H3, while it has no activity on monomethylated H3K9 residues.