Recombinant FBXL10 / KDM2B (accession number NP_115979.3) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 155.9 kDa.
KDM2B
宿主: 小鼠
宿主: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, SDS
应用备注
Recombinant FBXL10 / KDM2B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K36me2 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 300 nM Recombinant FBXL10 / KDM2B protein, and 3.3 μM H3K36me2 peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
限制
仅限研究用
注意事项
Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
储存条件
-80 °C
储存方法
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
KDM2B (lysine (K)-specific demethylase 2B), also known as FBXL10 (F-box and leucine-rich repeat protein 10) is a histone demethylase that preferentially demethylates trimethylated lysine 4 (K4me3) and dimethylated lysine 36 (K36me2) of histone H3. KDM2B displays weak or no activity for mono- and trimethylated H3K36. KDM2B preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which results in inhibition of cell growth and proliferation. KDM2B may also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.