NF-kB p65 Protein (C-Term, full length)
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- 抗原 See all NF-kB p65 (NFkBP65) 蛋白
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- 蛋白类型
- Recombinant
- 产品特性
- C-Term, full length
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Western Blotting (WB), ELISA
- 产品特性
- Recombinant NFκB p65 protein was expressed from a full-length cDNA clone in E. coli and has a 14 amino acid truncation at the C-term. This clone had five point mutations compared to the p65 sequence listed under accession no. AAA36408: L159V, P180S, F309S, A439V and V462M. The protein was purified by affinity chromatography, followed by gel filtration.
- 纯化方法
- Purified by affinity chromatography, followed by gel filtration.
- Top Product
- Discover our top product NFkBP65 蛋白
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Nuclear Factor-kB p65 (NFkBP65) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)
NFkBP65 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
Nuclear Factor-kB p65 (NFkBP65) (AA 1-220) protein (GST tag)NFkBP65 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nuclear Factor-kB p65 (NFkBP65) (AA 432-505) protein (GST tag)NFkBP65 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Recombinant NFκB p65 is suitable for Western blotting and TransAM® assays. 100 ng is sufficient for most protein-protein interaction studies. The standard curve for TransAM NFκB p65 was generated using the range of 40-0.625 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
- 限制
- 仅限研究用
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- 浓度
- 100 ng/μL
- 注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 储存条件
- -20 °C/-80 °C
- 储存方法
- Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- 抗原
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- 别名
- NFkappaB p65 (NFkBP65 产品)
- 别名
- C-Rel Protein, NFKB3 Protein, p65 Protein, NFkB Protein, c-rel Protein, zgc:100833 Protein, Xrel2 Protein, Xrel3 Protein, rel Protein, rel-A Protein, rel2 Protein, rel3 Protein, v-rel Protein, xrel Protein, REL proto-oncogene, NF-kB subunit Protein, RELA proto-oncogene, NF-kB subunit Protein, v-rel reticuloendotheliosis viral oncogene homolog A (avian) Protein, v-rel avian reticuloendotheliosis viral oncogene homolog Protein, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog Protein, REL Protein, RELA Protein, Rela Protein, Rel Protein, rel Protein, rel.L Protein
- 背景
- NFκB p65 is a subunit of the NFκB transcription factor complex that plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. NFκB p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains, they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFκB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IκB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IκB proteins. This releases NFκB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes.
- 途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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