Cathepsin E Protein (CTSE) (AA 18-396)
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- 抗原 See all Cathepsin E (CTSE) 蛋白
- Cathepsin E (CTSE)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 18-396
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Intracellular Flow Cytometry (ICFC)
- 纯度
- >95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Cathepsin E (CTSE) (AA 18-396) protein (GST tag)
CTSE 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin E (CTSE) (AA 60-397) protein (His tag)CTSE 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Cathepsin E (CTSE) (AA 1-397) protein (His tag)CTSE 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
Cathepsin E (CTSE) protein (His tag)CTSE 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Cathepsin E (CTSE) (AA 21-397) protein (His tag)CTSE 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS
Cathepsin E (CTSE) (AA 57-363) protein (His tag)CTSE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: After auto-activation, CTSE activity is determined by its ability to cleave the fluorogenic peptide substrate, Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2. The specific activity is >1,500 pmol/min/μg.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening.
- 缓冲液
- 0.22 μm filtered protein solution is in 20 mM MES, 150 mM NaCl, pH 6.5.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -70°C for six months.
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- 抗原
- Cathepsin E (CTSE)
- 别名
- Cathepsin E (CTSE 产品)
- 别名
- CATE Protein, A430072O03Rik Protein, C920004C08Rik Protein, CE Protein, CatE Protein, CEA Protein, CEB Protein, Ctsea Protein, CTSE Protein, ce1 Protein, ce2 Protein, cate Protein, CE1 Protein, ce1-A Protein, ctse Protein, CE2 Protein, ce2-A Protein, cathepsin E Protein, cathepsin E L homeolog Protein, cathepsin E S homeolog Protein, CTSE Protein, Ctse Protein, TP03_0056 Protein, Smp_184930 Protein, ctse Protein, ctse.L Protein, ctse.S Protein
- 背景
- Cathepesin E (CTSE) is an intracellular aspartic protease that was originally identified as a cathepsin D-like acid protease. CTSE and CTSD have similar substrate specificities and CTSE is active in acidic conditions in a pH range from 2.5 to 5.5. In vitro experiments have identified several CTSE substrates including insulin beta chain, neurokinin, and FGF. Although the function of CTSE is not completely understood, it has been implicated in several physiological and pathological processes. CTSE is required for antigen presentation on class II MHC molecules and subsequently, CTSE-deficient mice have increased susceptibility to bacterial infections. CTSE-deficient macrophages also show abnormalities, such as autophagy. Like many other cathepsins, CTSE has emerged as a therapy target for cancers, such as pancreatic ductal adenocarcinoma (PDAC). In addition to PDAC, CTSE is also overexpressed in gastric carcinomas and cervical and lung adenocarcinomas. The possible involvement of CTSE in neurodegeneration has also been reported.
- 分子量
- The 389 amino acid recombinant protein has a predicted molecular mass of approximately 42.3 kDa. The DTT-reduced protein migrates at approximately 45 kDa and the non-reduced protein migrates at approximately 90 kDa by SDS-PAGE. The N-terminal amino acid i
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