CCL25 Protein (AA 24-144)
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- 抗原 See all CCL25 蛋白
- CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 24-144
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
- 纯度
- > 98 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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- Discover our top product CCL25 蛋白
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Chemokine (C-C Motif) Ligand 25 (CCL25) (AA 24-113) protein (GST tag)
CCL25 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-C Motif) Ligand 25 (CCL25) (Transcript Variant 1) (Active) proteinCCL25 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, PI, STD Active
Chemokine (C-C Motif) Ligand 25 (CCL25) (AA 24-150) protein (GST tag)CCL25 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Bioactivity was measured by its ability to chemoattract Baf3-hCCR9 cells in a dose dependent manner.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))
- 别名
- CCL25 (CCL25 产品)
- 别名
- CCL25 Protein, Ckb15 Protein, SCYA25 Protein, TECK Protein, A130072A22Rik Protein, AI852536 Protein, CKb15 Protein, Scya25 Protein, C-C motif chemokine ligand 25 Protein, C-C motif chemokine 25 Protein, chemokine (C-C motif) ligand 25 Protein, Ccl25 Protein, CCL25 Protein, ccl25 Protein
- 背景
- CCL25, which signals through CCR9 receptor, is mainly expressed in the thymus and small intestine. In the thymus, CCL25 contributes to correct thymocyte maturation by promoting CD4+CD8+ thymocyte trafficking through the cortex via α4β1-dependent adhesion to VCAM-1, and also stimulates CD4+CD8- cell attachment to fibronectin in the medulla. In the small intestine, CCL25 is expressed by intestinal epithelium and controls segmental specialization of the intestinal immune system by recruiting gut-specific memory/effector T cells. CCL25 also promotes melanoma, ovarian cancer, breast cancer, leukemia, and prostatic carcinoma cell migration. A recent report shows that multiple myeloma cells can produce CCL25 and induce mesenchymal stromal cell migration. Bone marrow pre-pro-B cells also migrate in response to CCL25. CCL25 expression can be induced in the liver of patients with primary sclerosing cholangitis. Intestinal epithelial cell expression of CCL25 is also reduced in HIV-positive individuals.
- 分子量
- The 121 amino acid recombinant protein has a predicted molecular mass of approximately 16 kDa. The DTT-reduced and non-reduced protein migrate at approximately 16 kDa by SDS-PAGE. The N-terminal amino acid is Glutamine.
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