CCL25 Protein (AA 24-150)
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- 抗原 See all CCL25 蛋白
- CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 24-150
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 98 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Chemokine (C-C Motif) Ligand 25 (CCL25) (AA 24-113) protein (GST tag)
CCL25 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-C Motif) Ligand 25 (CCL25) (Transcript Variant 1) (Active) proteinCCL25 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, PI, STD Active
Chemokine (C-C Motif) Ligand 25 (CCL25) (AA 24-150) protein (GST tag)CCL25 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Bioactivity was measured by its property to chemoattract BaF3-hCCR9 transfectants in a dose dependent manner.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))
- 别名
- CCL25 (CCL25 产品)
- 别名
- CCL25 Protein, Ckb15 Protein, SCYA25 Protein, TECK Protein, A130072A22Rik Protein, AI852536 Protein, CKb15 Protein, Scya25 Protein, C-C motif chemokine ligand 25 Protein, C-C motif chemokine 25 Protein, chemokine (C-C motif) ligand 25 Protein, Ccl25 Protein, CCL25 Protein, ccl25 Protein
- 背景
- CCL25 (TECK) binds to CCR9, the only known receptor for TECK. CCR9 is expressed by a small population of circulating memory CD4+ and CD8+ lymphocytes expressing the intestinal homing receptor α4β7. Therefore, CCL25 is a key regulator for CCR9+CD4+ α4β7 T cell homing to the small intestine. In addition, CCL25 induces adhesion of CD103+ (αEβ7) on CD8 intraepithelial lymphocytes to its epithelial ligand E-cadherin. In inflammatory bowel diseases (IBD), as in celiac and Crohn's diseases, the proportion of CCR9+ cells in the peripheral blood and in the gut seems to be inversed, there is a lower frequency of CCR9+CD4+ T cells in the gut mucosa than in the peripheral blood. Also, a decrease in CCR9+CD4+ T cells occurs in the gut of HIV-infected patients. This decrease correlates with a lower expression of CCL25 by inflamed gut endothelial cells. Unusual expression of CCL25 in the liver occurs in primary sclerosis cholangitis (extra-intestinal complication of IBD). In this way, human T cells activated in the gut can be recruited to liver endothelium. Functional expression of CCR9 has been detected in melanoma derived cells, and it has been associated to small intestine metastatic melanoma processes. In addition, CCR9 is expressed by ovarian, breast, and prostate cancer cells.
- 分子量
- The 128 amino acid recombinant protein has a predicted molecular mass of approximately 14.3 kDa. The DTT-reduced and non-reduced protein migrate at approximately 16 and 17 kDa by SDS-PAGE respectively. The N-terminal amino acid is Met.
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