IL-34 Protein (AA 1-235)
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- 抗原 See all IL-34 (IL34) 蛋白
- IL-34 (IL34) (Interleukin 34 (IL34))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-235
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宿主
- 小鼠
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资源
- CHO Cells
- 应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)
- 纯度
- > 98 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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- Discover our top product IL34 蛋白
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Interleukin 34 (IL34) protein
IL34 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
Interleukin 34 (IL34) protein (Myc-DYKDDDDK Tag)IL34 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: ED50 =10 - 50 ng/ml, corresponding to a specific activity of 0.2 - 1 x 105 units/mg, as determined by M-NFS-60 cell proliferation induction in a dose dependent manner.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- 缓冲液
- 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 0.5 M NaCl, pH 7.4
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- 抗原
- IL-34 (IL34) (Interleukin 34 (IL34))
- 别名
- IL-34 (IL34 产品)
- 别名
- C16orf77 Protein, IL-34 Protein, 2010004A03Rik Protein, AI593503 Protein, C18H16ORF77 Protein, interleukin 34 Protein, IL34 Protein, Il34 Protein
- 背景
- Mouse IL-34 shares a sequence identity of 71 % with human IL-34 on the amino acid level, and the IL-34 gene is syntenic in the human, chimpanzee, rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif and does not share sequence homology with M-CSF, nevertheless, it binds to the CSFR. These two cytokines are not identical in biological activity and signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines have different ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests a differential domain binding in the receptor to IL-34 and CSF. As a result, different bioactivities and signal activation kinetics/strength are produced for these cytokines.
- 分子量
- The 215 amino acid recombinant protein has a predicted molecular mass of 26 kDa. The DTT-reduced protein migrates at approximately 39 kDa and the non-reduced protein with slightly greater mobility by SDS-PAGE.
- 途径
- RTK signaling
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