Biological activity was determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 1.0-2.0 μg/mL.
纯度
< 95 % by SDS-PAGE gel and HPLC analyses.
内毒素水平
Endotoxin level is less than 0.1 ng per μg (1 EU/μg).
As with any protein, exposing BMP-5 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
储存条件
-20 °C
储存方法
The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted BMP-5 stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
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背景
TGF-beta family members are key modulators of cell proliferation, differentiation, matrix synthesis, and apoptosis. As implied by their name, BMPs initiate, promote, and regulate the development, growth and remodeling of bone and cartilage. In addition to this role, BMPs are also involved in prenatal development and postnatal growth, remodeling and maintenance of a variety of other tissues and organs. BMP-5 is expressed in the nervous system, lung and liver. It is a known regulator for dendritic growth in sympathetic neurons. BMP-5 is a 454 amino acid precursor protein that is cleaved to release the biologically active C-terminal mature protein. Recombinant human BMP-5 is a 31.2 kDa homodimeric disulfide-linked protein consisting of two identical 138 amino acid chains.