Taq SSB (Active) 蛋白
宿主: Thermus aquaticus
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % of protein determined by SDS-PAGE (CBB staining)
Func
Active
(1 reference)-
- 抗原
- Taq SSB
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 宿主
- Thermus aquaticus
- 资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func)
- 产品特性
- The absence of endonucleases and exonucleases was confirmed.
- 纯度
- > 95 % of protein determined by SDS-PAGE (CBB staining)
-
- 应用备注
- Stabilizes single-stranded DNA in DNA replication,repair,and recombination
- 说明
-
Activity: Single-stranded DNA binding activity was confirmed.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 50 mM Tris-HCl ( pH 8. 0), 200 mM NaCl, 0. 1 mM dithiothreitol, 0. 5 mM EDTA, 50 % glycerol
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
-
-
: "Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification." in: Protein expression and purification, Vol. 26, Issue 1, pp. 131-8, (2002) (PubMed).
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: "Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification." in: Protein expression and purification, Vol. 26, Issue 1, pp. 131-8, (2002) (PubMed).
-
- 抗原
- Taq SSB
- 背景
- Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA. It plays important roles in DNA replication and recombination. Thermus aquaticus SSB gene was expressed in E.Coli in large quantities and the protein was highly purified. MW is 30.0 kDa, same as that of the natural protein.
- UniProt
- Q9KH06
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