Streptolysin O Protein (SLO)
SLO
宿主: Streptococcus pyogenes
宿主: 大肠杆菌（E. Coli）
Recombinant
> 98 % by SDS-PAGE
ELISA, WB, SDS
Active
(3 references)-
- 抗原 See all Streptolysin O (SLO) 蛋白
- Streptolysin O (SLO)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- Streptococcus pyogenes
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 产品特性
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Measurement of the activity: Definition of 1HU is activation of 50 % hemolysis using 3 % sheep red blood cells.
The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci. The specific activity is as high as 1,900,000 hemolytic units (HU) /mg and the product forms a big hole on the cell membrane, which enables the introduction of protein inside the cells in vivo. - 纯度
- > 98 % by SDS-PAGE
- 过滤
- Sterile filtered
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- 应用备注
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1) Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent
2) Reagent for membrane pore formation to introduce small-to-macromolecules into living cells - 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Inactivated SLO can be reactivated by thiol reagents such as 20 mM cysteine or 10 mM DTT (2)
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 1 mM DTT, 50 % glycerol
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C/-80 °C
- 储存方法
- -20 C (For long term storage: -70 C)
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: "GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus." in: Nature cell biology, Vol. 10, Issue 10, pp. 1135-45, (2008) (PubMed).
: "Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 6, pp. 3185-90, (2001) (PubMed).
: "The family of thiol-activated, cholesterol-binding cytolysins." in: Toxicon : official journal of the International Society on Toxinology, Vol. 39, Issue 11, pp. 1681-9, (2001) (PubMed).
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: "GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus." in: Nature cell biology, Vol. 10, Issue 10, pp. 1135-45, (2008) (PubMed).
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- 抗原
- Streptolysin O (SLO)
- 别名
- Streptolysin O (SLO 产品)
- 背景
- Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cells. SLO is easily inactivated in the presence of oxygen but can be reactivated by thiol compounds, so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98 %.
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