电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

RNASEH 蛋白

RNASEH 宿主: E. coli 宿主: 大肠杆菌(E. Coli) Recombinant > 95 % protein determined by SDS-PAGE (CBB staining) Active
产品编号 ABIN3200996
发货至: 中国
  • 抗原 See all RNASEH 蛋白
    RNASEH (Ribonuclease H (RNASEH))
    蛋白类型
    Recombinant
    生物活性
    Active
    宿主
    • 2
    • 1
    • 1
    E. coli
    资源
    • 4
    大肠杆菌(E. Coli)
    产品特性
    Specific Activity: 100, 000 units/mg protein
    Unit Definition: 1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37 C.
    纯度
    > 95 % protein determined by SDS-PAGE (CBB staining)
  • 应用备注
    1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA.
    2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT)
    3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    50 units/L
    缓冲液
    50 units/ul in 20 mM Tris-HCl ( pH 7.5), 100 mM KCl, 1 mM DTT, 50 % Glycerol
    储存液
    Dithiothreitol (DTT)
    注意事项
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
  • Gubler: "Second-strand cDNA synthesis: mRNA fragments as primers." in: Methods in enzymology, Vol. 152, pp. 330-5, (1987) (PubMed).

    Donis-Keller: "Site specific enzymatic cleavage of RNA." in: Nucleic acids research, Vol. 7, Issue 1, pp. 179-92, (1979) (PubMed).

    Vournakis, Efstratiadis, Kafatos: "Electrophoretic patterns of deadenylylated chorion and globin mRNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, Issue 8, pp. 2959-63, (1976) (PubMed).

  • 抗原
    RNASEH (Ribonuclease H (RNASEH))
    Abstract
    RNASEH 产品
    别名
    BA1623 Protein, rnhA Protein, SC5H1.08c Protein, ribonuclease H Protein, ribonuclease HI Protein, Ribonuclease H Protein, rnhA Protein, SCO7284 Protein, CBU_0316 Protein, CNA03470 Protein, CNN02200 Protein, Tb10.70.2140 Protein, Tmath_1295 Protein, Deima_2376 Protein, Tresu_0232 Protein, Marky_0915 Protein, Desac_1125 Protein, Corgl_1288 Protein
    背景
    Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact. E.coli RNase H (=RNaseHI) was over-expressed in E. coli as a recombinant protein and highly purified. MW is 17.6 kDa.
    UniProt
    P0A7Y4
You are here:
客服