Recombinant Bacteriophage Host-nuclease inhibitor protein gam is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val138) of Bacteriophage GAM fused with a 6His tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
限制
仅限研究用
状态
Lyophilized
溶解方式
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.4.
储存液
Dithiothreitol (DTT)
注意事项
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
抗原
Host-Nuclease Inhibitor Protein Gam
背景
Host-nuclease inhibitor protein gam(gam) comes from Enterobacteria phage lambda. Originally the gam gene product was assigned to the sequence shown and it was suggested that the gene kil protein is encoded from within this gene and corresponds to residues 41-138. For an alternative assignment of this gene see the entry for the kil gene protein of lambda. The function of the protein inhibits the host recBC-coded nuclease and thus promotes the late, rolling-circle mode of lambda DNA replication. Synonyms: Host-nuclease inhibitor protein gam,gamma