TEV Protease 蛋白
宿主: Tobacco Etch Virus (TEV)
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % by SDS-PAGE analyses.
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- 抗原
- TEV Protease
- 蛋白类型
- Recombinant
- 宿主
- Tobacco Etch Virus (TEV)
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资源
- 大肠杆菌（E. Coli）
- 产品特性
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6 IU/μl
Unit Definition: One unit of TEV protease cleaves > 85 % of 3 μg of control substrate in 1 hour at pH 8.0 at 30 °C. - 纯度
- > 95 % by SDS-PAGE analyses.
- 过滤
- Sterile
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Sterile liquid solution contains 50 mM Tris, 5 mM DTT, 50 % glycerol, pH 7.5.
- 注意事项
- Avoid freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20 °C from date of receipt.
- 有效期
- 12 months
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- 抗原
- TEV Protease
- 物质类
- Viral Protein
- 背景
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Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30 °C, also it can be used at temperature as low as 4 °C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques.
Synonyms: rTEV, TEV, P1 protease - 分子量
- 28.4 kDa, observed by reducing SDS-PAGE.
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