Sonic Hedgehog Protein (SHH) (AA 24-197, Cys24IleIle-Mutant)
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- 抗原 See all Sonic Hedgehog (SHH) 蛋白
- Sonic Hedgehog (SHH)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 24-197, Cys24IleIle-Mutant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 产品特性
- ED50 < 2.0 μg/mL, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of > 500 units/mg.
- 纯度
- > 95 % by SDS-PAGE and HPLC analyses.
- 内毒素水平
- < 0.2 EU/μg, determined by LAL method.
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Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) protein
SHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 24-197) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE SDS
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Sonic Hedgehog (SHH) (AA 48-197) protein (His-IF2DI Tag)SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) proteinSHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstituted in ddH2O at 100 μg/mL.
- 缓冲液
- Lyophilized after extensive dialysis against PBS.
- 储存条件
- -80 °C
- 储存方法
- Lyophilized recombinant human Sonic Hedgehog (C24II) (rhShh) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhShh should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- 有效期
- 6 months
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- 抗原
- Sonic Hedgehog (SHH)
- Abstract
- SHH 产品
- 别名
- HHG1 Protein, HLP3 Protein, HPE3 Protein, MCOPCB5 Protein, SMMCI Protein, TPT Protein, TPTPS Protein, 9530036O11Rik Protein, Dsh Protein, Hhg1 Protein, Hx Protein, Hxl3 Protein, M100081 Protein, fc83d08 Protein, shh Protein, syu Protein, vhh-1 Protein, vhh1 Protein, wu:fc83d08 Protein, Xhh Protein, hedgehog Protein, xshh Protein, SHH Protein, twh Protein, twhh Protein, sonic hedgehog Protein, sonic hedgehog a Protein, sonic hedgehog L homeolog Protein, sonic hedgehog protein A Protein, sonic hedgehog b Protein, SHH Protein, Shh Protein, shha Protein, shh.L Protein, shh Protein, shhb Protein
- 背景
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Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are three related Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93 % identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.Recombinant human Sonic Hedgehog (C24II) (rhShh) produced in E. coli is a single non-glycosylated polypeptide chain containing 175 amino acids. A fully biologically active molecule, rhShh has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE.
Synonyms: Hhg1 - 分子量
- 19.7 kDa, observed by reducing SDS-PAGE.
- UniProt
- Q15465
- 途径
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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