The EC50 value of rat MCP-1/CCL2 on Ca2+ mobilization assay in CHO-K1/G alpha 15/rCCR2 cells (human G alpha 15 and rat CCR2 stably expressed in CHO-K1 cells) is less than 0.3 μg/mL.
CCL2
宿主: 小鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
> 98 % pure
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstituted in ddH2O or PBS at 100 μg/mL.
缓冲液
Lyophilized after extensive dialysis against PBS.
储存条件
-80 °C
储存方法
Lyophilized recombinant Rat MCP-1/CCL2 remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, Rat MCP-1/CCL2 should be stable up to 1 week at 4 °C or up to 3 months at -20 °C.
Chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemotactic protein 1 (MCP1) and small inducible cytokine A2. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. CCL2 is implicated in the pathogeneses of several diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis. CCL2 is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. CCL2 is primarily secreted by monocytes, macrophages and dendritic cells. CCL2 can signal through the CCR2 receptor.Recombinant rat MCP-1/CCL2 produced in HEK293 cells is a polypeptide chain containing 125 amino acids. A fully biologically active molecule, rrMCP-1/CCL2 has a molecular mass of 10-15 kDa analyzed by reducing SDS-PAGE. Synonyms: MCP-1, MCAF, JE, Monocyte Chemotactic Protein-1 (MCP-1), CCL2