ED50 < 0.2 μg/mL, measured by a cell proliferation assay of HUVEC cells in the presence of 2.5 ng/mL h-VEGF, corresponding to a specific activity of > 5.0x 10^3 IU/mg. AA 22-98, expressed with an N-terminal Met.
CXCL10
宿主: Cynomolgus
宿主: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0.
储存条件
-80 °C
储存方法
Lyophilized recombinant human IP-10/CXCL10 (rhIP-10) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhIP-10 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
IP-10/CXCL10 also known as CXCL10, is originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that IP-10 mRNA is also induced by LPS, IL-1beta, TNF-alpha, IL-12 and viruses. Additional cell types that have been shown to express IP-10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. IP-10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human IP-10, Crg-2, has been cloned and shown to share approximately 67 % amino acid sequence identity with human IP-10.Recombinant human IP-10/CXCL10 (rhIP-10) produced in E. coli is a single non-glycosylated polypeptide chain containing 78 amino acids. A fully biologically active molecule, rhIP-10 has a molecular mass of 8.6 kDa analyzed by reducing SDS-PAGE. Synonyms: Small inducible cytokine B10, CXCL10, 10 kDa interferon-gamma-induced protein, Gamma-IP10, IP-10, chemokine (C-X-C motif) ligand 10, C7, IFI10, INP10, crg-2, mob-1, SCYB10, gIP-10