ED50 <0.1 ng/mL, measured by the dose-dependent stimulation of the proliferation of IL-6 dependent murine 7TD1 cells, corresponding to a specific activity of > 1 x 10^7 units/mg. AA 29-212, expressed with an N-terminal Met.
IL6
宿主: 犬
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % by SDS-PAGE gel analyses
WB, ELISA
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstituted in ddH2O at 100 μg/mL.
缓冲液
Lyophilized after extensive dialysis against 1xPBS.
储存条件
-80 °C
储存方法
Lyophilized recombinant human Interleukin-6 (rhIL-6) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhIL-6 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
Interleukin-6 (IL-6, also known as IFN-beta2) is a pleiotropic cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 is secreted by T cells and macrophages to stimulate immune response. IL-6 is responsible for stimulating acute phase protein synthesis, as well as the production of neutrophils in the bone marrow. It supports the growth of B cells and is antagonistic to regulatory T cells.Recombinant human Interleukin-6 (rhIL-6) produced in E. coli is a single non-glycosylated polypeptide chain containing 185 amino acids. A fully biologically active molecule, rhIL-6 has a molecular mass of 20.9 kDa analyzed by reducing SDS-PAGE. Synonyms: 26 kDa protein, IFN-beta2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2