ED50 < 0.5 ng/mL, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 2 x 10^7 units/mg. AA 20-152, expressed with an N-terminal Met.
IL-3
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % as determined by SDS-PAGE
WB, ELISA
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstituted in ddH2O at 100 μg/mL.
缓冲液
Lyophilized after extensive dialysis against PBS.
储存条件
-80 °C
储存方法
Lyophilized recombinant human Interleukin-3 (rhIL-3) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhIL-3 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a four-helix bundle structure, are located on the same chromosomes in both human and mouse, are produced by activated T cells, and share receptors. The IL-3/IL-5/GM-CSF receptor family members are all heterodimeric, composed of a receptor-specific alpha chain and a common beta chain. IL-3 is also called multi-colony stimulating factor since it stimulates the development and colony formation of multiple lineages of hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. IL-3 inhibits apoptosis and promotes cell survival by targeting the anti-apoptotic bcl-2 gene family. Recombinant human Interleukin-3 (rhIL-3) produced in E. coli is a single non-glycosylated polypeptide chain containing of 134 amino acids. A fully biologically active molecule, rhIL-3 has a molecular mass of 15.2 kDa analyzed by non-reducing SDS-PAGE. Synonyms: MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor