ED50 < 0.5 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 2.0x 10^6 units/mg. AA 10 -154, expressed with an N-terminal Met.
FGF2
宿主: Pig
宿主: 大肠杆菌(E. Coli)
Recombinant
> 98 % by SDS-PAGE
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstituted in ddH2O at 100 μg/mL.
缓冲液
Lyophilized after extensive dialysis against PBS.
储存条件
-80 °C
储存方法
Lyophilized recombinant mouse Fibroblast Growth Factor-basic (rmFGF-basic) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmFGF-basic should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
Fibroblast Growth Factor-basic (FGF-basic), also known as HBGF-2, is a non-glycosylated heparin-binding growth factor that belongs to the FGF family. FGF-basic is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. FGF-basic signals through FGFR1, 2, 3 and 4 that plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration.Recombinant mouse Fibroblast Growth Factor-basic (rmFGF-basic) produced in E. coli is a single non-glycosylated polypeptide chain containing 146 amino acids. A fully biologically active molecule, rmFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE. Synonyms: Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin, BFGF, FGFB,