ED50 < 0.25 ng/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4 x 10^6 units/mg. AA 10-154, expressed with an N-terminal Gly.
FGF2
宿主: Pig
宿主: 大肠杆菌(E. Coli)
Recombinant
> 98 % by SDS-PAGE
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstituted in ddH2O at 100 μg/mL.
缓冲液
Lyophilized after extensive dialysis against PBS.
储存条件
-80 °C
储存方法
Lyophilized recombinant rat Fibroblast Growth Factor-basic (rrFGF-basic) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrFGF-basic remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the beta trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.Recombinant rat Fibroblast Growth Factor-basic (rrFGF-basic) produced in E. coli is a single non-glycosylated polypeptide chain containing 146 amino acids. A fully biologically active molecule, rrFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE. Synonyms: Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin