EGF Protein (AA 977-1029)
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- 抗原 See all EGF 蛋白
- EGF (Epidermal Growth Factor (EGF))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 977-1029
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宿主
- 小鼠
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资源
- 大肠杆菌(E. Coli)
- 产品特性
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ED50 < 0.1 ng/mL, measured by a cell proliferation assay using BALB/c 3T3 cells, corresponding to a specific activity of > 1.0x 10^7 units/mg.
AA 977-1029, expressed with an N-terminal Met. - 纯度
- > 95 % by SDS-PAGE and HPLC analysis.
- 内毒素水平
- < 0.2 EU/μg, determined by LAL method.
- Top Product
- Discover our top product EGF 蛋白
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstituted in ddH2O at 100 μg/mL.
- 缓冲液
- Lyophilized after extensive dialysis against PBS.
- 储存条件
- -80 °C
- 储存方法
- Lyophilized recombinant mouse Epidermal Growth Factor (rmEGF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmEGF should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- 有效期
- 6 months
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- 抗原
- EGF (Epidermal Growth Factor (EGF))
- Abstract
- EGF 产品
- 别名
- HOMG4 Protein, URG Protein, AI790464 Protein, CEGF Protein, epidermal growth factor Protein, pro-epidermal growth factor Protein, EGF Protein, egf Protein, CpipJ_CPIJ020278 Protein, Egf Protein
- 背景
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Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor).Recombinant mouse Epidermal Growth Factor (rmEGF) produced in E. coli is a single non-glycosylated polypeptide chain containing 54 amino acids. A fully biologically active molecule, rmEGF is obtained by proprietary chromatographic techniques with a molecular mass of 6.2 kDa analyzed by reducing SDS-PAGE.
Synonyms: Epidermal Growth Factor, Urogastrone, URG - 分子量
- 6.2 kDa, observed by reducing SDS-PAGE.
- UniProt
- P01132
- 途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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