TMPRSS15 蛋白
TMPRSS15
宿主: Cow
宿主: Yeast (Pichia pastoris)
Recombinant
> 95 % by SDS-PAGE analyses.
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- 抗原 See all TMPRSS15 蛋白
- TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))
- 蛋白类型
- Recombinant
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宿主
- Cow
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资源
- Yeast (Pichia pastoris)
- 产品特性
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5 IU/μl
Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95 % completion at 22 °C in a buffer containing 25 mM Tris-HCl, pH 8.0. - 纯度
- > 95 % by SDS-PAGE analyses.
- 过滤
- Sterile
- 内毒素水平
- < 1.0 EU/μg, determined by LAL method.
- 组件
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100 IU (or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
100 μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0)
3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl2) Storage: Store kit components at -20 °C.
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Transmembrane Protease, serine 15 (TMPRSS15) (AA 785-10199) protein
TMPRSS15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Sterile liquid solution contians 20 mM Tris, 200 mM NaCl, 2 mM CaCl2, 50 % glycerol, pH 7.4.
- 注意事项
- Avoid freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20 °C from date of receipt. It will remain stable at 37 °C for one week without losing any activity.
- 有效期
- 12 months
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- 抗原
- TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))
- 别名
- Enterokinase(EK) (TMPRSS15 产品)
- 背景
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Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques.
Synonyms: Enteropeptidase, ENTK, PRSS7 - 分子量
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Theoretical MW: 22.7 kDa.
Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.
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