desert Hedgehog Protein (AA 23-198, Cys23IleIle-Mutant)
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- 抗原 See all desert Hedgehog (DHH) 蛋白
- desert Hedgehog (DHH) (Desert Hedgehog (DHH))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 23-198, Cys23IleIle-Mutant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 产品特性
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ED50 < 10 μg/, measured by its ability to induce alkaline phosphatase production by CCL-226 cells, corresponding to a specific activity of > 100 units/mg.
AA 23-198 (Cys23IleIle), with and without an N-terminal Met - 纯度
- > 95 % by SDS-PAGE and HPLC analyses.
- 内毒素水平
- < 0.2 EU/μg, determined by LAL method.
- Top Product
- Discover our top product DHH 蛋白
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Desert Hedgehog (DHH) (Active) protein
DHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Desert Hedgehog (DHH) (AA 23-198) protein (His tag)DHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Desert Hedgehog (DHH) (AA 24-198) protein (His tag)DHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Desert Hedgehog (DHH) (AA 24-198) protein (His tag)DHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Desert Hedgehog (DHH) (AA 50-320) protein (His tag)DHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Desert Hedgehog (DHH) (AA 1-396) protein (GST tag)DHH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstituted in ddH2O at 100 μg/mL.
- 缓冲液
- Lyophilized after extensive dialysis against PBS.
- 储存条件
- -80 °C
- 储存方法
- Lyophilized recombinant Human Hedgehog homolog(rhDHH) remains stable up to 6 months at-80 °C from date of receipt. Upon reconstitution, rhDHH remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- 有效期
- 6 months
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- 抗原
- desert Hedgehog (DHH) (Desert Hedgehog (DHH))
- Abstract
- DHH 产品
- 别名
- GDXYM Protein, HHG-3 Protein, SRXY7 Protein, C78960 Protein, Xchh Protein, chh Protein, chh-a Protein, dhh Protein, dhh-1 Protein, dhh-A Protein, hh4 Protein, x-chh Protein, desert hedgehog Protein, desert hedgehog L homeolog Protein, desert hedgehog S homeolog Protein, DHH Protein, dhh Protein, Dhh Protein, dhh.L Protein, dhh.S Protein
- 物质类
- Species
- 背景
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Desert hedgehog protein (DHH) is a member of the Hedgehog family which encodes signaling molecules that play an important role in regulating morphogenesis. It is predicted to be made as a precursor that is auto-catalytically cleaved, the N-terminal portion is soluble and contains the signaling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. DHH may be involved in both male gonadal differentiation and perineurial development. DHH binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip). It induces steroidogenic factor 1(SF1), which is instrumental in promoting Leydig cell differentiation. It also promotes the deposition of basal lamina surrounding seminiferous tubules. Recombinant human Desert hedgehog (DHH) produced in Escherichia Coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule, rhDHH has a molecular mass of around 20 kDa analyzed by reducing SDS-PAGE.
Synonyms: DHH, Human - 分子量
- 20 kDa, observed by reducing SDS-PAGE.
- 途径
- Hedgehog Signaling
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