BMP2 Protein (AA 283-396)
(15 references)-
- 抗原 See all BMP2 蛋白
- BMP2 (Bone Morphogenetic Protein 2 (BMP2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 283-396
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 产品特性
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ED50 < 1 μg/mL, measured by alkaline phosphatase induction assay using C2C12 cells.
AA 283-396, expressed with an N-terminal Met. - 纯度
- > 95 % as analyzed by non-reducing SDS-PAGE.
- 内毒素水平
- < 1 EU/μg, determined by LAL method.
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Bone Morphogenetic Protein 2 (BMP2) (AA 283-396) protein (His-IF2DI Tag)
BMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Bone Morphogenetic Protein 2 (BMP2) (AA 283-396) proteinBMP2 宿主: 人, 犬, 小鼠, 大鼠, 猕猴 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Bone Morphogenetic Protein 2 (BMP2) (AA 1-396) protein (GST tag)BMP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Bone Morphogenetic Protein 2 (BMP2) (AA 231-330) protein (GST tag)BMP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Bone Morphogenetic Protein 2 (BMP2) (AA 283-396) protein (GST tag)BMP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Bone Morphogenetic Protein 2 (BMP2) (AA 283-396) protein (Fc Tag)BMP2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Bone Morphogenetic Protein 2 (BMP2) (AA 284-396) proteinBMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >92 % as determined by SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstituted in 20 mM AcOH or 5 mM HCl. The solubility should be at 100 μg/mL.
- 缓冲液
- Lyophilized after extensive dialysis against 50 mM acetic acid.
- 储存条件
- -80 °C
- 储存方法
- Lyophilized recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhBMP-2 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- 有效期
- 6 months
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: "BMP-2 inhibits tumor-initiating ability in human renal cancer stem cells and induces bone formation." in: Journal of cancer research and clinical oncology, Vol. 141, Issue 6, pp. 1013-24, (2015) (PubMed).
: "Segmental mandibular bone reconstruction with a carbonate-substituted hydroxyapatite-coated modular endoprosthetic poly(?-caprolactone) scaffold in Macaca fascicularis." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 102, Issue 5, pp. 962-76, (2014) (PubMed).
: "Mechanical properties and dual drug delivery application of poly(lactic-co-glycolic acid) scaffolds fabricated with a poly(?-amino ester) porogen." in: Acta biomaterialia, Vol. 10, Issue 5, pp. 2125-32, (2014) (PubMed).
: "Growth factor priming differentially modulates components of the extracellular matrix proteome in chondrocytes and synovium-derived stem cells." in: PLoS ONE, Vol. 9, Issue 2, pp. e88053, (2014) (PubMed).
: "Simple signaling molecules for inductive bone regenerative engineering." in: PLoS ONE, Vol. 9, Issue 7, pp. e101627, (2014) (PubMed).
: "Tendon progenitor cells in injured tendons have strong chondrogenic potential: the CD105-negative subpopulation induces chondrogenic degeneration." in: Stem cells (Dayton, Ohio), Vol. 32, Issue 12, pp. 3266-77, (2014) (PubMed).
: "Caveolae regulate Smad signaling as verified by novel imaging and system biology approaches." in: Journal of cellular physiology, Vol. 228, Issue 5, pp. 1060-9, (2013) (PubMed).
: "BMP-2 inhibits tumor growth of human renal cell carcinoma and induces bone formation." in: International journal of cancer. Journal international du cancer, Vol. 131, Issue 8, pp. 1941-50, (2012) (PubMed).
: "A novel library screen identifies immunosuppressors that promote osteoblast differentiation." in: Bone, Vol. 50, Issue 6, pp. 1294-303, (2012) (PubMed).
: "Etanercept does not impair healing in rat models of tendon or metaphyseal bone injury." in: Acta orthopaedica, Vol. 83, Issue 3, pp. 305-10, (2012) (PubMed).
: "The role of histone chaperones in osteoblastic differentiation of C2C12 myoblasts." in: Biochemical and biophysical research communications, Vol. 423, Issue 4, pp. 726-32, (2012) (PubMed).
: "Lithium chloride attenuates BMP-2 signaling and inhibits osteogenic differentiation through a novel WNT/GSK3- independent mechanism." in: Bone, Vol. 48, Issue 2, pp. 321-31, (2011) (PubMed).
: "Potent inhibition of heterotopic ossification by nuclear retinoic acid receptor-? agonists." in: Nature medicine, Vol. 17, Issue 4, pp. 454-60, (2011) (PubMed).
: "Affinity-based growth factor delivery using biodegradable, photocrosslinked heparin-alginate hydrogels." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 154, Issue 3, pp. 258-66, (2011) (PubMed).
: "Casein kinase 2 regulates in vivo bone formation through its interaction with bone morphogenetic protein receptor type Ia." in: Bone, Vol. 49, Issue 5, pp. 944-54, (2011) (PubMed).
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: "BMP-2 inhibits tumor-initiating ability in human renal cancer stem cells and induces bone formation." in: Journal of cancer research and clinical oncology, Vol. 141, Issue 6, pp. 1013-24, (2015) (PubMed).
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- 抗原
- BMP2 (Bone Morphogenetic Protein 2 (BMP2))
- 别名
- Bone Morphogenetic Protein-2 (BMP-2) (BMP2 产品)
- 别名
- BDA2 Protein, BMP2A Protein, AI467020 Protein, Bmp2a Protein, BMP-2 Protein, xBMP-2 Protein, xbmp2 Protein, BMP2 Protein, bmp2a Protein, bmp2 Protein, wu:fc59d09 Protein, bone morphogenetic protein 2 Protein, bone morphogenetic protein 2 L homeolog Protein, Bone morphogenetic protein 2 Protein, bone morphogenetic protein 2a Protein, BMP2 Protein, Bmp2 Protein, bmp2.L Protein, bmp2 Protein, bmp2a Protein
- 背景
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Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecule (Met1-Arg396, with a signal peptide from Met1 to Gly23), propeptide (Leu24-Arg282) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). The active form consists of a dimer of two identical proteins which are linked by a disulfide bond at Cys360. It plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction. Recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 115 amino acids. A fully biologically active molecule, rhBMP-2 has a molecular mass of 26 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: BMP-2, BMP2A, Bone morphogenetic protein 2, BMP-2A, BMP2 - 分子量
- 26 kDa, observed by non-reducing SDS-PAGE
- UniProt
- P12643
- 途径
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation
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