Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/mL.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
缓冲液
Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 7.4, 500 mM NaCl.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
4 °C/-20 °C
储存方法
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The a-defensins are distinguished from the b-defensins by the pairing of their three disulfide bonds. To date, four rat b-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. The b-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. b-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney. Synonyms: Beta-defensin 4, BD-4, BD-2, Defensin beta 4, RBD-2, RBD-4, Defb4, Defb2, Defb3.
分子量
4.4 kDa, a single non-glycosylated polypeptide chain containing 41 amino acids.