ED50 < 5 μg/mL, measured by a cell growth inhibitory assay using M1 cells, corresponding to a specific activity of > 2x 10^2 units/mg. AA 19-244, expressed with an N-terminal Met.
ADIPOQ
宿主: 人
宿主: 小麦胚
Recombinant
WB, ELISA, AP, AA
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstituted in ddH2O at 100 μg/mL.
缓冲液
Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.
储存条件
-80 °C
储存方法
Lyophilized recombinant human Adiponectin (rhAdiponectin) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhAdiponectin remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
Adiponectin is a hormone mainly produced by adipocytes. Adiponectin forms a homotrimer and exists as higher order multimers in vivo. The receptors of Adiponectin are seven-transmembrane G protein coupled receptors: Receptor 1 is expressed in skeletal muscle and Receptor 2 in liver. Adiponectin receives a lot of attention because of its anti-diabetic, anti-atherosclerotic, and anti-inflammatory properties. Adiponectin increases the expression of molecules involved in fatty acid transport, combustion of fatty acid, and energy dissipation, and increases insulin sensitivity of the body. Decreased levels of Adiponectin are associated with hypertension, cardiovascular diseases, and metabolic syndromes. Therefore, Adiponectin has promising potential as a pharmacological agent. Recombinant human Adiponectin (rhAdiponectin) produced in E. coli is a single non-glycosylated polypeptide chain containing 227 amino acids. A fully biologically active molecule, rhAdiponectin has a molecular mass of 24.7 kDa analyzed by reducing SDS-PAGE. Synonyms: Acrp-30, GBP-28, Apm-1