Recombinant Human Semaphorin-4B/SEMA4B is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu39-Glu712) of Human SEMA4B fused with a polyhistidine tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
SEMA4B
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
限制
仅限研究用
状态
Liquid
溶解方式
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
-80 °C
储存方法
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
有效期
6 months
抗原
SEMA4B
(Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4B (SEMA4B))
The class 4 semaphorins are integral membrane proteins that are widely expressed throughout the nervous system. SEMA4B is a single-pass type I membrane protein and contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one Sema domain. Human SEMA4B is expressed in neurons. SEMA4B inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. SEMA4B negatively regulates basophil-mediated Th2 and humoral memory responses.